9FQ6

Structure of the complete Vaccinia DNA-dependent RNA polymerase complex assembly intermediate 4

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA-directed RNA polymerase 147 kDa polypeptide	polymer	1286	146995.7	1	UniProt (P20504) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Vaccinia virus
2	B (B)	DNA-directed RNA polymerase 133 kDa polypeptide	polymer	1164	133526.9	1	UniProt (P68694) Pfam (PF04560) Pfam (PF12415) Pfam (PF04565) Pfam (PF04567) Pfam (PF00562)	Vaccinia virus
3	C (C)	DNA-directed RNA polymerase 35 kDa subunit	polymer	305	35430.7	1	UniProt (P21087) Pfam (PF03396)	Vaccinia virus
4	D (E)	DNA-directed RNA polymerase 22 kDa subunit	polymer	185	21365.7	1	UniProt (P68608) Pfam (PF05273)	Vaccinia virus
5	E (F)	DNA-directed RNA polymerase 19 kDa subunit	polymer	164	19020.1	1	UniProt (Q76ZQ8) Pfam (PF05320) UniProt (by SIFTS) (P68610)	Vaccinia virus
6	F (G)	DNA-directed RNA polymerase 18 kDa subunit	polymer	161	17917.2	1	UniProt (P21034) Pfam (PF03293)	Vaccinia virus
7	G (I)	RNA polymerase-associated transcription-specificity factor RAP94	polymer	795	93667.6	1	UniProt (P68439) Pfam (PF03294)	Vaccinia virus	Protein H4,RPO-associated protein of 94 kDa
8	H (J)	DNA-directed RNA polymerase 7 kDa subunit	polymer	63	7299.7	1	UniProt (P68315)	Vaccinia virus
9	I (L)	mRNA-capping enzyme regulatory subunit OPG124	polymer	287	33396.7	1	UniProt (P20980) Pfam (PF03341)	Vaccinia virus	Virus termination factor small subunit,VTF small subunit,mRNA-capping enzyme 33 kDa subunit,mRNA-capping enzyme D12 subunit,mRNA-capping enzyme small subunit
10	J (O)	mRNA-capping enzyme catalytic subunit	polymer	844	96888.8	1	UniProt (P20979) Pfam (PF10640) Pfam (PF21004) Pfam (PF21005) Pfam (PF03291)	Vaccinia virus	Virus termination factor large subunit,VTF large subunit,mRNA-capping enzyme 97 kDa subunit,mRNA-capping enzyme D1 subunit,mRNA-capping enzyme large subunit
11	K, L (Q, R)	Core protein OPG073	polymer	129	14914.1	2	UniProt (P68449) Pfam (PF06138)	Vaccinia virus	Protein E11
12	M (S)	DNA-directed RNA polymerase 30 kDa polypeptide	polymer	259	30074.3	1	UniProt (P21082) Pfam (PF12410) Pfam (PF01096)	Vaccinia virus
13	N (U)	URQ-UUG1-1 tRNA (72-MER)	polymer	72	23150.7	1		Vaccinia virus
14	O (Y)	Nucleoside triphosphatase I	polymer	631	72465.3	1	UniProt (P20637) UniProt (by SIFTS) (Q49PL2)	Vaccinia virus	Factor X,NPH-I,Nucleoside triphosphate phosphohydrolase I,NPH I
15	P, U, V, W (A, U)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
16	Q, R, S, T (A, B, I)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
17	AA, BA, CA, DA, X... (I, L, S, Y, A...)	water	water		18.0	11	Chemie (HOH)

Sequence modifications

A: 1 - 1286 (UniProt: P20504)

PDB	External Database	Details
Thr 258	Ser 258	variant
Glu 489	Lys 489	variant
Lys 1015	Arg 1015	variant

B: 1 - 1164 (UniProt: P68694)

PDB	External Database	Details
Asn 6	Asp 6	variant
Phe 343	Tyr 343	variant

C: 1 - 305 (UniProt: P21087)

PDB	External Database	Details
Asn 236	Asp 236	variant

L: 1 - 287 (UniProt: P20980)

PDB	External Database	Details
Asn 235	Asp 235	variant

O: 1 - 844 (UniProt: P20979)

PDB	External Database	Details
Phe 8	Ser 8	variant
Thr 202	Lys 202	variant

Y: 1 - 631 (UniProt: P20637)

PDB	External Database	Details
Asp 329	Asn 329	variant

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	761027.5
Non-Polymers*	Number of molecules	8
	Total formula weight	358.9
All*	Total formula weight	761386.4

*Water molecules are not included.