9FPY
Structure of the complete Vaccinia DNA-dependent RNA polymerase complex assembly intermediate 3
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | DNA-directed RNA polymerase 35 kDa subunit | polymer | 305 | 35430.7 | 1 | UniProt (P21087) Pfam (PF03396) | Orthopoxvirus vaccinia | |
| 2 | B (E) | DNA-directed RNA polymerase 22 kDa subunit | polymer | 185 | 21365.7 | 1 | UniProt (P68608) Pfam (PF05273) | Orthopoxvirus vaccinia | |
| 3 | C (G) | DNA-directed RNA polymerase 18 kDa subunit | polymer | 161 | 17917.2 | 1 | UniProt (P21034) Pfam (PF03293) | Orthopoxvirus vaccinia | |
| 4 | D (J) | DNA-directed RNA polymerase 7 kDa subunit | polymer | 63 | 7299.7 | 1 | UniProt (P68315) Pfam (PF05864) | Orthopoxvirus vaccinia | |
| 5 | E, F (Q, R) | Core protein OPG073 | polymer | 129 | 14914.1 | 2 | UniProt (P68449) Pfam (PF06138) | Orthopoxvirus vaccinia | Protein E11 |
| 6 | G (U) | URQ-UUG1-1 tRNA (72-MER) | polymer | 72 | 23150.7 | 1 | Vaccinia virus | ||
| 7 | H (A) | DNA-directed RNA polymerase 147 kDa polypeptide | polymer | 1286 | 146995.7 | 1 | UniProt (P20504) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Orthopoxvirus vaccinia | |
| 8 | I (B) | DNA-directed RNA polymerase 133 kDa polypeptide | polymer | 1164 | 133526.9 | 1 | UniProt (P68694) Pfam (PF04560) Pfam (PF12415) Pfam (PF04565) Pfam (PF04567) Pfam (PF00562) | Orthopoxvirus vaccinia | |
| 9 | J (F) | DNA-directed RNA polymerase 19 kDa subunit | polymer | 164 | 19020.1 | 1 | UniProt (Q76ZQ8) Pfam (PF05320) UniProt (by SIFTS) (P68610) | Orthopoxvirus vaccinia | |
| 10 | K (I) | RNA polymerase-associated transcription-specificity factor RAP94 | polymer | 795 | 93667.6 | 1 | UniProt (P68439) Pfam (PF03294) | Orthopoxvirus vaccinia | Protein H4,RPO-associated protein of 94 kDa |
| 11 | L (S) | DNA-directed RNA polymerase 30 kDa polypeptide | polymer | 259 | 30074.3 | 1 | UniProt (P21082) Pfam (PF12410) Pfam (PF01096) | Orthopoxvirus vaccinia | |
| 12 | M (Y) | Nucleoside triphosphatase I | polymer | 631 | 72465.3 | 1 | UniProt (P20637) Pfam (PF00176) Pfam (PF00271) Pfam (PF08469) | Orthopoxvirus vaccinia | Factor X,NPH-I,Nucleoside triphosphate phosphohydrolase I,NPH I |
| 13 | N, O, P, U (U, A, Y) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 14 | Q, R, S, T (A, B, I) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 15 | AA, V, W, X, Y... (Y, A, B, F, I...) | water | water | 18.0 | 9 | Chemie (HOH) |
Sequence modifications
C: 1 - 305 (UniProt: P21087)
A: 1 - 1286 (UniProt: P20504)
B: 1 - 1164 (UniProt: P68694)
Y: 1 - 631 (UniProt: P20637)
| PDB | External Database | Details |
|---|---|---|
| Asn 236 | Asp 236 | variant |
| PDB | External Database | Details |
|---|---|---|
| Thr 258 | Ser 258 | variant |
| Glu 489 | Lys 489 | variant |
| Lys 1015 | Arg 1015 | variant |
| PDB | External Database | Details |
|---|---|---|
| Asn 6 | Asp 6 | variant |
| Phe 343 | Tyr 343 | variant |
| PDB | External Database | Details |
|---|---|---|
| Asp 329 | Asn 329 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 630742.1 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 358.9 | |
| All* | Total formula weight | 631101.0 |
