9FP3
Ap4A bound in de novo transcription initiation T. thermophilus RNA polymerase complex with TC DNA template
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 315 | 35056.2 | 2 | UniProt (Q5SHR6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Thermus thermophilus HB8 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1119 | 125436.5 | 1 | UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Thermus thermophilus HB8 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1524 | 170997.4 | 1 | UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Thermus thermophilus HB8 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 99 | 11533.3 | 1 | UniProt (Q8RQE7) Pfam (PF01192) | Thermus thermophilus HB8 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | RNA polymerase sigma factor SigA | polymer | 449 | 51470.1 | 1 | UniProt (Q72L95) | Thermus thermophilus HB8 | |
| 6 | G (G) | DNA (31-MER) | polymer | 53 | 16375.5 | 1 | synthetic construct | ||
| 7 | H (H) | DNA (35-MER) | polymer | 53 | 16450.5 | 1 | synthetic construct | ||
| 8 | I, M, N, O (C, D) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) PubChem (888) | |||
| 9 | J (C) | BIS(ADENOSINE)-5'-TETRAPHOSPHATE | non-polymer | 836.4 | 1 | Chemie (B4P) PubChem (24867496) PubChem (44286171) PubChem (54592217) PubChem (58298222) PubChem (194) PubChem (6328499) PubChem (21706) PubChem (10795591) PubChem (13455593) PubChem (118705482) PubChem (6603812) PubChem (11513435) PubChem (11957522) PubChem (13455592) PubChem (146121073) PubChem (171904579) | |||
| 10 | K, L (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 11 | P (G) | PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER | non-polymer | 521.2 | 1 | Chemie (G2P) |
Sequence modifications
F: 1 - 423 (UniProt: Q72L95)
| PDB | External Database | Details |
|---|---|---|
| Met -25 | - | initiating methionine |
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Gly -16 | - | expression tag |
| Val -15 | - | expression tag |
| Asp -14 | - | expression tag |
| Leu -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Thr -11 | - | expression tag |
| Glu -10 | - | expression tag |
| Asn -9 | - | expression tag |
| Leu -8 | - | expression tag |
| Tyr -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Ser -4 | - | expression tag |
| Asn -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 462375.7 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1585.6 | |
| All* | Total formula weight | 463961.4 |
