9FP0

Cryo-EM structure of the 'crown'less Bcs macrocomplex for E. coli cellulose secretion in non-saturating c-di-GMP (local)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (D, E)	Cyclic di-GMP binding protein BcsE	polymer	536	60967.5	2	Pfam (PF10995) UniProt (by SIFTS) (P37657)	Escherichia coli
2	C, E (Q, W)	Cell division protein	polymer	250	27960.8	2	UniProt (A0A0B1KWQ0) Pfam (PF06564) UniProt (by SIFTS) (P0DP92)	Escherichia coli	Cellulose biosynthesis protein BcsQ,Cellulose synthase operon protein YhjQ,Cellulose synthase,putative
3	D, K (R, V)	Protein YhjR	polymer	77	8645.5	2	Pfam (PF10945) UniProt (by SIFTS) (P0ADJ3)	Escherichia coli
4	F (A)	Cellulose synthase catalytic subunit [UDP-forming]	polymer	908	104015.7	1	Pfam (PF00535) Pfam (PF07238) UniProt (by SIFTS) (P37653)	Escherichia coli
5	G, H, I (J, K, G)	Cellulose biosynthesis protein BcsG	polymer	536	59688.0	3	Pfam (PF11658) UniProt (by SIFTS) (P37659)	Escherichia coli
6	J (B)	Cyclic di-GMP-binding protein	polymer	779	86184.4	1	UniProt (A0A061KLG7) Pfam (PF03170) UniProt (by SIFTS) (P37652)	Escherichia coli	Cellulose synthase regulatory subunit
7	L, M (F, S)	Cellulose biosynthesis protein BcsF	polymer	63	7379.0	2	Pfam (PF11120) UniProt (by SIFTS) (P0ADJ5)	Escherichia coli
8	N, O, P, Q (D, E)	9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)	non-polymer		690.4	4	Chemie (C2E)
9	R, T (Q, W)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
10	S, U (Q, W)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	579169.8
Non-Polymers*	Number of molecules	8
	Total formula weight	3824.6
All*	Total formula weight	582994.4

*Water molecules are not included.