9FNN

Cryo-EM structure of the c-di-GMP-saturated 'crown'less Bcs macrocomplex for cellulose secretion in E. coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cellulose synthase catalytic subunit [UDP-forming]	polymer	908	104015.7	1	Pfam (PF00535) Pfam (PF07238) UniProt (by SIFTS) (P37653)	Escherichia coli
2	B, K, L, M (B, X, Y, Z)	Cyclic di-GMP-binding protein	polymer	779	86184.4	4	UniProt (A0A061KLG7) Pfam (PF03170) UniProt (by SIFTS) (P37652)	Escherichia coli	Cellulose synthase regulatory subunit
3	C, H (E, U)	Cyclic di-GMP binding protein BcsE	polymer	536	60967.5	2	Pfam (PF10995) UniProt (by SIFTS) (P37657)	Escherichia coli
4	D, G (F, S)	Cellulose biosynthesis protein BcsF	polymer	63	7379.0	2	Pfam (PF11120) UniProt (by SIFTS) (P0ADJ5)	Escherichia coli
5	E, J (Q, W)	Cell division protein	polymer	250	27960.8	2	UniProt (A0A0B1KWQ0) Pfam (PF06564) UniProt (by SIFTS) (P0DP92)	Escherichia coli	Cellulose biosynthesis protein BcsQ,Cellulose synthase operon protein YhjQ,Cellulose synthase,putative
6	F, I (R, V)	Protein YhjR	polymer	77	8645.5	2	Pfam (PF10945) UniProt (by SIFTS) (P0ADJ3)	Escherichia coli
7	N, O (G, D)	Cellulose biosynthesis protein BcsG	polymer	536	59688.0	2	Pfam (PF11658) UniProt (by SIFTS) (P37659)	Escherichia coli
8	P, Q, R, S, V... (A, E, U)	9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)	non-polymer		690.4	6	Chemie (C2E)
9	T, X (Q, W)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
10	U, Y (Q, W)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	778035.0
Non-Polymers*	Number of molecules	10
	Total formula weight	5205.4
All*	Total formula weight	783240.4

*Water molecules are not included.