9FL8

Stapled peptide bound to NOT9-NOT1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (G, F)	Protein bag of marbles	polymer	19	2063.5	2	UniProt (P22745)	Drosophila melanogaster	CAF40-binding motif domain peptide
2	C, E (B, C)	CCR4-NOT transcription complex subunit 9	polymer	273	31072.2	2	UniProt (Q92600) Pfam (PF04078)	Homo sapiens (human)	Cell differentiation protein RQCD1 homolog,Rcd-1
3	D, F (A, D)	CCR4-NOT transcription complex subunit 1	polymer	244	27617.9	2	UniProt (A5YKK6) Pfam (PF12842)	Homo sapiens (human)	CCR4-associated factor 1,Negative regulator of transcription subunit 1 homolog,hNOT1
4	G, H, I, J, K... (F, B, A, C)	1,4-BUTANEDIOL	non-polymer		90.1	6	Chemie (BU1)
5	M, N, O, P, Q (G, B, A, C, D)	water	water		18.0	46	Chemie (HOH)

Sequence modifications

G, F: 17 - 33 (UniProt: P22745)

PDB	External Database	Details
Ace 16	-	acetylation
Mk8 19	His 19	engineered mutation
Mk8 23	Gln 23	engineered mutation
Nle 24	Met 24	engineered mutation
Nle 31	Met 31	engineered mutation
Nh2 34	-	amidation

B, C: 19 - 285 (UniProt: Q92600)

PDB	External Database	Details
Gly 13	-	expression tag
Pro 14	-	expression tag
His 15	-	expression tag
Met 16	-	expression tag
Leu 17	-	expression tag
Glu 18	-	expression tag

A, D: 1351 - 1588 (UniProt: A5YKK6)

PDB	External Database	Details
Gly 1345	-	expression tag
Pro 1346	-	expression tag
His 1347	-	expression tag
Met 1348	-	expression tag
Leu 1349	-	expression tag
Glu 1350	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	121507.2
Non-Polymers*	Number of molecules	6
	Total formula weight	540.7
All*	Total formula weight	122047.9

*Water molecules are not included.