9FIL
Crystal Structure of reduced NuoEF variant E222K(NuoF) from Aquifex aeolicus bound to NAD+
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | NADH-quinone oxidoreductase subunit E | polymer | 160 | 18573.6 | 2 | UniProt (O66842) Pfam (PF01257) In PDB | Aquifex aeolicus VF5 | NADH dehydrogenase I subunit E,NDH-1 subunit E |
2 | B, D | NADH-quinone oxidoreductase subunit F | polymer | 434 | 48523.4 | 2 | UniProt (O66841) Pfam (PF01512) Pfam (PF22461) Pfam (PF10531) Pfam (PF10589) In PDB | Aquifex aeolicus VF5 | NADH dehydrogenase I subunit F,NDH-1 subunit F |
3 | A, C | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
4 | D, A, B, C | SODIUM ION | non-polymer | 23.0 | 10 | Chemie (NA) | |||
5 | B, D | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
6 | B, D | 1-DEOXY-1-(7,8-DIMETHYL-2,4-DIOXO-3,4-DIHYDRO-2H-BENZO[G]PTERIDIN-1-ID-10(5H)-YL)-5-O-PHOSPHONATO-D-RIBITOL | non-polymer | 458.4 | 2 | Chemie (FNR) | |||
7 | B, D | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 2 | Chemie (NAD) | |||
8 | B, D | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
9 | B, D | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
10 | water | water | 18.0 | 418 | Chemie (HOH) |
Sequence modifications
B, D: 1 - 426 (UniProt: O66841)
PDB | External Database | Details |
---|---|---|
Lys 222 | Glu 222 | engineered mutation |
Ala 427 | - | expression tag |
Gly 428 | - | expression tag |
His 429 | - | expression tag |
His 430 | - | expression tag |
His 431 | - | expression tag |
His 432 | - | expression tag |
His 433 | - | expression tag |
His 434 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 134194.0 | |
Non-Polymers* | Number of molecules | 24 |
Total formula weight | 3911.0 | |
All* | Total formula weight | 138105.0 |