9FID

X-ray structure of furin (PCSK3) in complex with the PC1/3 (PCSK1) prodomain mutant R78K,R80A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, B, C)	Furin	polymer	482	52388.6	3	UniProt (P09958) Pfam (PF00082) Pfam (PF01483)	Homo sapiens (human)	Dibasic-processing enzyme,Paired basic amino acid residue-cleaving enzyme,PACE
2	B, D, F (D, E, F)	Neuroendocrine convertase 1	polymer	95	11069.3	3	UniProt (P29120) Pfam (PF16470)	Homo sapiens (human)	NEC 1,Prohormone convertase 1,Proprotein convertase 1,PC1
3	G, H, J, K, M... (A, B, C)	CALCIUM ION	non-polymer		40.1	6	Chemie (CA)
4	I, L, O (A, B, C)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
5	P, Q, R, S, T... (A, D, B, E, C...)	water	water		18.0	106	Chemie (HOH)

Sequence modifications

A, B, C: 108 - 574 (UniProt: P09958)

PDB	External Database	Details
Ser 575	-	expression tag
Gly 576	-	expression tag
Ser 577	-	expression tag
Leu 578	-	expression tag
Val 579	-	expression tag
Pro 580	-	expression tag
Arg 581	-	expression tag
Gly 582	-	expression tag
Ser 583	-	expression tag
His 584	-	expression tag
His 585	-	expression tag
His 586	-	expression tag
His 587	-	expression tag
His 588	-	expression tag
His 589	-	expression tag

D, E, F: 28 - 110 (UniProt: P29120)

PDB	External Database	Details
Met 16	-	initiating methionine
Gly 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
Ser 24	-	expression tag
Gly 25	-	expression tag
His 26	-	expression tag
Met 27	-	expression tag
Lys 78	Arg 78	engineered mutation
Ala 80	Arg 80	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	190373.8
Non-Polymers*	Number of molecules	9
	Total formula weight	309.4
All*	Total formula weight	190683.3

*Water molecules are not included.