9FI5

Bacteroides ovatus polysaccharide lyase family 38 (BoPL38) mutant Y298F in complex tetramannuronic acid at pH 3.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Alginate lyase family protein	polymer	404	45956.2	4	UniProt (A0A5M5BWR5) Pfam (PF05426)	Bacteroides ovatus
2	E, F, G, H (E, F, G, H)	beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid	branched		722.5	4	In PDB GlyTouCan (G72539QT)
3	I, J, K, L (A, B, D)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
4	M, N, O, P (A, B, C, D)	water	water		18.0	418	Chemie (HOH)

Sequence modifications

A, B, C, D: 24 - 404 (UniProt: A0A5M5BWR5)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Leu 14	-	expression tag
Val 15	-	expression tag
Pro 16	-	expression tag
Arg 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
His 20	-	expression tag
Met 21	-	expression tag
Ala 22	-	expression tag
Ser 23	-	expression tag
Phe 298	Tyr 294	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	183824.9
Branched	Number of molecules	4
	Total formula weight	2890.0
Non-Polymers*	Number of molecules	4
	Total formula weight	384.3
All*	Total formula weight	187099.2

*Water molecules are not included.