9FGC
Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to one PIP2 molecule at chain alpha1-D and in complex with Mb38
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | Gamma-aminobutyric acid receptor subunit alpha-1 | polymer | 464 | 52916.6 | 2 | UniProt (P14867) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit alpha-1 |
| 2 | B, E (B, E) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 473 | 54444.6 | 2 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit beta-3 |
| 3 | C (C) | Gamma-aminobutyric acid receptor subunit gamma-2 | polymer | 495 | 56922.1 | 1 | UniProt (P18507) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit gamma-2 |
| 4 | F (F) | Megabody-38,Outer membrane protein | polymer | 539 | 57784.3 | 1 | UniProt (B5Z8H1) Pfam (PF18304) | Lama glama | |
| 5 | G (a) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 1 | In PDB GlyTouCan (G40702WU) | |||
| 6 | H, K (b, e) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
| 7 | I, L (G, H) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 2 | In PDB GlyTouCan (G09724ZC) | |||
| 8 | J (d) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 9 | M (A) | DECANE | non-polymer | 142.3 | 1 | Chemie (D10) | |||
| 10 | N (C) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 11 | O (D) | [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate | non-polymer | 746.6 | 1 | Chemie (PIO) |
Sequence modifications
A, D: 1 - 429 (UniProt: P14867)
C: -38 - 436 (UniProt: P18507)
F: 2 - 13 (PDB: 9FGC)
F: 14 - 237 (UniProt: B5Z8H1)
F: 238 - 540 (PDB: 9FGC)
| PDB | External Database | Details |
|---|---|---|
| Met -34 | - | initiating methionine |
| Lys -33 | - | expression tag |
| Lys -32 | - | expression tag |
| Ser -31 | - | expression tag |
| Pro -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Leu -28 | - | expression tag |
| Ser -27 | - | expression tag |
| Asp -26 | - | expression tag |
| Tyr -25 | - | expression tag |
| Leu -24 | - | expression tag |
| Trp -23 | - | expression tag |
| Ala -22 | - | expression tag |
| Trp -21 | - | expression tag |
| Thr -20 | - | expression tag |
| Leu -19 | - | expression tag |
| Phe -18 | - | expression tag |
| Leu -17 | - | expression tag |
| Ser -16 | - | expression tag |
| Thr -15 | - | expression tag |
| Leu -14 | - | expression tag |
| Thr -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Arg -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Tyr -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Asp -7 | - | expression tag |
| Tyr -6 | - | expression tag |
| Lys -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Lys 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 437 | - | expression tag |
| Gly 438 | - | expression tag |
| Ser 439 | - | expression tag |
| Gly 440 | - | expression tag |
| Gly 441 | - | expression tag |
| Ser 442 | - | expression tag |
| Gly 443 | - | expression tag |
| Gly 444 | - | expression tag |
| Ser 445 | - | expression tag |
| Gly 446 | - | expression tag |
| Lys 447 | - | expression tag |
| Thr 448 | - | expression tag |
| Glu 449 | - | expression tag |
| Thr 450 | - | expression tag |
| Ser 451 | - | expression tag |
| Gln 452 | - | expression tag |
| Val 453 | - | expression tag |
| Ala 454 | - | expression tag |
| Pro 455 | - | expression tag |
| Ala 456 | - | expression tag |
F: 14 - 237 (UniProt: B5Z8H1)
F: 238 - 540 (PDB: 9FGC)
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 329428.7 | |
| Branched | Number of molecules | 6 |
| Total formula weight | 5627.1 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1110.1 | |
| All* | Total formula weight | 336165.9 |
