9FG0
Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric open 2 state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | Gamma-aminobutyric acid receptor subunit alpha-1 | polymer | 338 | 38791.7 | 2 | UniProt (P14867) | Homo sapiens (human) | GABA(A) receptor subunit alpha-1,GABAAR subunit alpha-1 |
| 2 | B, E (B, E) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 333 | 38719.8 | 2 | UniProt (P28472) | Homo sapiens (human) | GABA(A) receptor subunit beta-3,GABAAR subunit beta-3 |
| 3 | C (C) | Gamma-aminobutyric acid receptor subunit gamma-2 | polymer | 334 | 38753.6 | 1 | UniProt (P18507) | Homo sapiens (human) | GABA(A) receptor subunit gamma-2,GABAAR subunit gamma-2 |
| 4 | F (F) | Nanobody38 | polymer | 123 | 13492.9 | 1 | Lama glama | ||
| 5 | G (a) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 1 | In PDB GlyTouCan (G40702WU) | |||
| 6 | H, J, L (b, c, e) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 7 | I, M (G, H) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 2 | In PDB GlyTouCan (G09724ZC) | |||
| 8 | K (d) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 9 | N, O (B, E) | GAMMA-AMINO-BUTANOIC ACID | non-polymer | 103.1 | 2 | Chemie (ABU) |
Sequence modifications
A, D: 10 - 312 (UniProt: P14867)
A, D: 391 - 418 (UniProt: P14867)
B, E: 8 - 307 (UniProt: P28472)
B, E: 422 - 447 (UniProt: P28472)
C: 25 - 322 (UniProt: P18507)
C: 400 - 428 (UniProt: P18507)
A, D: 391 - 418 (UniProt: P14867)
| PDB | External Database | Details |
|---|---|---|
| Ser 313 | - | linker |
| Gln 314 | - | linker |
| Pro 315 | - | linker |
| Ala 316 | - | linker |
| Arg 317 | - | linker |
| Ala 318 | - | linker |
| Ala 319 | - | linker |
B, E: 422 - 447 (UniProt: P28472)
| PDB | External Database | Details |
|---|---|---|
| Ser 308 | - | linker |
| Gln 309 | - | linker |
| Pro 310 | - | linker |
| Ala 311 | - | linker |
| Arg 312 | - | linker |
| Ala 313 | - | linker |
| Ala 314 | - | linker |
C: 400 - 428 (UniProt: P18507)
| PDB | External Database | Details |
|---|---|---|
| Ser 323 | - | linker |
| Gln 324 | - | linker |
| Pro 325 | - | linker |
| Ala 326 | - | linker |
| Arg 327 | - | linker |
| Ala 328 | - | linker |
| Gly 429 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 207269.6 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 6051.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 206.2 | |
| All* | Total formula weight | 213527.3 |
