9FFY
Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric open1 state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | Gamma-aminobutyric acid receptor subunit alpha-1 | polymer | 411 | 47059.6 | 2 | UniProt (P14867) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit alpha-1,GABAAR subunit alpha-1 |
| 2 | B, E (B, E) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 395 | 45643.2 | 2 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit beta-3,GABAAR subunit beta-3 |
| 3 | C (C) | Isoform 1 of Gamma-aminobutyric acid receptor subunit gamma-2 | polymer | 373 | 42957.0 | 1 | UniProt (P18507) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit gamma-2,GABAAR subunit gamma-2 |
| 4 | F (F) | Nanobody38 | polymer | 133 | 14748.2 | 1 | Lama glama | ||
| 5 | G (a) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 1 | In PDB GlyTouCan (G40702WU) | |||
| 6 | H, J, L (b, c, e) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 7 | I, M (G, H) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 2 | In PDB GlyTouCan (G09724ZC) | |||
| 8 | K (d) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 9 | N (A) | (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate | non-polymer | 749.0 | 1 | Chemie (D3D) | |||
| 10 | O, P (B, E) | GAMMA-AMINO-BUTANOIC ACID | non-polymer | 103.1 | 2 | Chemie (ABU) |
Sequence modifications
A, D: 5 - 312 (UniProt: P14867)
A, D: 391 - 429 (UniProt: P14867)
B, E: 1 - 307 (UniProt: P28472)
B, E: 422 - 448 (UniProt: P28472)
C: 1 - 322 (UniProt: P18507)
C: 400 - 428 (UniProt: P18507)
| PDB | External Database | Details |
|---|---|---|
| Met -52 | - | initiating methionine |
| Asp -51 | - | expression tag |
| Glu -50 | - | expression tag |
| Lys -49 | - | expression tag |
| Thr -48 | - | expression tag |
| Thr -47 | - | expression tag |
| Gly -46 | - | expression tag |
| Trp -45 | - | expression tag |
| Arg -44 | - | expression tag |
| Gly -43 | - | expression tag |
| Gly -42 | - | expression tag |
| His -41 | - | expression tag |
| Val -40 | - | expression tag |
| Val -39 | - | expression tag |
| Glu -38 | - | expression tag |
| Gly -37 | - | expression tag |
| Leu -36 | - | expression tag |
| Ala -35 | - | expression tag |
| Gly -34 | - | expression tag |
| Glu -33 | - | expression tag |
| Leu -32 | - | expression tag |
| Glu -31 | - | expression tag |
| Gln -30 | - | expression tag |
| Leu -29 | - | expression tag |
| Arg -28 | - | expression tag |
| Ala -27 | - | expression tag |
| Arg -26 | - | expression tag |
| Leu -25 | - | expression tag |
| Glu -24 | - | expression tag |
| His -23 | - | expression tag |
| His -22 | - | expression tag |
| Pro -21 | - | expression tag |
| Gln -20 | - | expression tag |
| Gly -19 | - | expression tag |
| Gln -18 | - | expression tag |
| Arg -17 | - | expression tag |
| Glu -16 | - | expression tag |
| Pro -15 | - | expression tag |
| Asp -14 | - | expression tag |
| Tyr -13 | - | expression tag |
| Asp -12 | - | expression tag |
| Ile -11 | - | expression tag |
| Pro -10 | - | expression tag |
| Thr -9 | - | expression tag |
| Thr -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Thr 0 | - | expression tag |
| Gly 1 | - | expression tag |
| Gln 2 | - | expression tag |
| Pro 3 | - | expression tag |
| Ser 4 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 313 | - | linker |
| Gln 314 | - | linker |
| Pro 315 | - | linker |
| Ala 316 | - | linker |
| Arg 317 | - | linker |
| Ala 318 | - | linker |
| Ala 319 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Met -53 | - | initiating methionine |
| Asp -52 | - | expression tag |
| Glu -51 | - | expression tag |
| Lys -50 | - | expression tag |
| Thr -49 | - | expression tag |
| Thr -48 | - | expression tag |
| Gly -47 | - | expression tag |
| Trp -46 | - | expression tag |
| Arg -45 | - | expression tag |
| Gly -44 | - | expression tag |
| Gly -43 | - | expression tag |
| His -42 | - | expression tag |
| Val -41 | - | expression tag |
| Val -40 | - | expression tag |
| Glu -39 | - | expression tag |
| Gly -38 | - | expression tag |
| Leu -37 | - | expression tag |
| Ala -36 | - | expression tag |
| Gly -35 | - | expression tag |
| Glu -34 | - | expression tag |
| Leu -33 | - | expression tag |
| Glu -32 | - | expression tag |
| Gln -31 | - | expression tag |
| Leu -30 | - | expression tag |
| Arg -29 | - | expression tag |
| Ala -28 | - | expression tag |
| Arg -27 | - | expression tag |
| Leu -26 | - | expression tag |
| Glu -25 | - | expression tag |
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| Pro -22 | - | expression tag |
| Gln -21 | - | expression tag |
| Gly -20 | - | expression tag |
| Gln -19 | - | expression tag |
| Arg -18 | - | expression tag |
| Glu -17 | - | expression tag |
| Pro -16 | - | expression tag |
| Asp -15 | - | expression tag |
| Tyr -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Ile -12 | - | expression tag |
| Pro -11 | - | expression tag |
| Thr -10 | - | expression tag |
| Thr -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Asn -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Thr -1 | - | expression tag |
| Gly 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 308 | - | linker |
| Gln 309 | - | linker |
| Pro 310 | - | linker |
| Ala 311 | - | linker |
| Arg 312 | - | linker |
| Ala 313 | - | linker |
| Ala 314 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Thr -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Thr 11 | Ala 50 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser 323 | - | linker |
| Gln 324 | - | linker |
| Pro 325 | - | linker |
| Ala 326 | - | linker |
| Arg 327 | - | linker |
| Ala 328 | - | linker |
| Gly 429 | - | expression tag |
| Thr 430 | - | expression tag |
| Gly 431 | - | expression tag |
| Gly 432 | - | expression tag |
| Thr 433 | - | expression tag |
| Thr 434 | - | expression tag |
| Glu 435 | - | expression tag |
| Thr 436 | - | expression tag |
| Ser 437 | - | expression tag |
| Gln 438 | - | expression tag |
| Val 439 | - | expression tag |
| Ala 440 | - | expression tag |
| Pro 441 | - | expression tag |
| Ala 442 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 243111.1 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 6051.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 955.2 | |
| All* | Total formula weight | 250117.8 |
