9FFN

Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric bound-closed 1 state of branch 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (D, A)	Gamma-aminobutyric acid receptor subunit alpha-1	polymer	411	47059.6	2	UniProt (P14867) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit alpha-1
2	B, C, E (E, B, C)	Gamma-aminobutyric acid receptor subunit beta-3	polymer	395	45643.2	3	UniProt (P28472) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit beta-3
3	F (F)	Megabody25,Outer membrane protein	polymer	522	56300.6	1	UniProt (B5Z8H1) Pfam (PF18304)	Lama glama
4	G, H, J, L, M (a, b, c, d, e)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	5	In PDB GlyTouCan (G22768VO)
5	I, K, N (G, H, I)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
6	O, Q, T (D, E)	DECANE	non-polymer		142.3	3	Chemie (D10)
7	P, V (D, A)	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate	non-polymer		760.1	2	Chemie (POV)
8	R, W (D, A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
9	S, U (E, B)	GAMMA-AMINO-BUTANOIC ACID	non-polymer		103.1	2	Chemie (ABU)

Sequence modifications

D, A: 5 - 312 (UniProt: P14867)

PDB	External Database	Details
Met -52	-	initiating methionine
Asp -51	-	expression tag
Glu -50	-	expression tag
Lys -49	-	expression tag
Thr -48	-	expression tag
Thr -47	-	expression tag
Gly -46	-	expression tag
Trp -45	-	expression tag
Arg -44	-	expression tag
Gly -43	-	expression tag
Gly -42	-	expression tag
His -41	-	expression tag
Val -40	-	expression tag
Val -39	-	expression tag
Glu -38	-	expression tag
Gly -37	-	expression tag
Leu -36	-	expression tag
Ala -35	-	expression tag
Gly -34	-	expression tag
Glu -33	-	expression tag
Leu -32	-	expression tag
Glu -31	-	expression tag
Gln -30	-	expression tag
Leu -29	-	expression tag
Arg -28	-	expression tag
Ala -27	-	expression tag
Arg -26	-	expression tag
Leu -25	-	expression tag
Glu -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
Pro -21	-	expression tag
Gln -20	-	expression tag
Gly -19	-	expression tag
Gln -18	-	expression tag
Arg -17	-	expression tag
Glu -16	-	expression tag
Pro -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag
Gly 1	-	expression tag
Gln 2	-	expression tag
Pro 3	-	expression tag
Ser 4	-	expression tag

D, A: 391 - 429 (UniProt: P14867)

PDB	External Database	Details
Ser 313	-	linker
Gln 385	-	linker
Pro 386	-	linker
Ala 387	-	linker
Arg 388	-	linker
Ala 389	-	linker
Ala 390	-	linker

E, B, C: 1 - 307 (UniProt: P28472)

PDB	External Database	Details
Met -53	-	initiating methionine
Asp -52	-	expression tag
Glu -51	-	expression tag
Lys -50	-	expression tag
Thr -49	-	expression tag
Thr -48	-	expression tag
Gly -47	-	expression tag
Trp -46	-	expression tag
Arg -45	-	expression tag
Gly -44	-	expression tag
Gly -43	-	expression tag
His -42	-	expression tag
Val -41	-	expression tag
Val -40	-	expression tag
Glu -39	-	expression tag
Gly -38	-	expression tag
Leu -37	-	expression tag
Ala -36	-	expression tag
Gly -35	-	expression tag
Glu -34	-	expression tag
Leu -33	-	expression tag
Glu -32	-	expression tag
Gln -31	-	expression tag
Leu -30	-	expression tag
Arg -29	-	expression tag
Ala -28	-	expression tag
Arg -27	-	expression tag
Leu -26	-	expression tag
Glu -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
Pro -22	-	expression tag
Gln -21	-	expression tag
Gly -20	-	expression tag
Gln -19	-	expression tag
Arg -18	-	expression tag
Glu -17	-	expression tag
Pro -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Ile -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Thr -1	-	expression tag
Gly 0	-	expression tag

E, B, C: 422 - 448 (UniProt: P28472)

PDB	External Database	Details
Ser 308	-	linker
Gln 309	-	linker
Pro 310	-	linker
Ala 311	-	linker
Arg 312	-	linker
Ala 313	-	linker
Ala 314	-	linker

F: 2 - 13 (PDB: 9FFN)
F: 14 - 234 (UniProt: B5Z8H1)
F: 235 - 403 (UniProt: B5Z8H1)
F: 404 - 523 (PDB: 9FFN)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	287349.7
Branched	Number of molecules	8
	Total formula weight	5827.3
Non-Polymers*	Number of molecules	9
	Total formula weight	2224.1
All*	Total formula weight	295401.1

*Water molecules are not included.