9FFM

Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with Mb25 in the resting state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (D, A)	Gamma-aminobutyric acid receptor subunit alpha-1	polymer	411	47059.6	2	UniProt (P14867) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit alpha-1
2	B, C, E (E, B, C)	Gamma-aminobutyric acid receptor subunit beta-3	polymer	395	45643.2	3	UniProt (P28472) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit beta-3
3	F (F)	Megabody25,Outer membrane protein	polymer	522	56300.6	1	UniProt (B5Z8H1)	Lama glama
4	G, H, J, L, M (a, b, c, d, e)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	5	In PDB GlyTouCan (G22768VO)
5	I, K, N (G, H, I)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
6	O, P, Q (E, B, C)	DECANE	non-polymer		142.3	3	Chemie (D10)

Sequence modifications

D, A: 5 - 312 (UniProt: P14867)

PDB	External Database	Details
Met -52	-	initiating methionine
Asp -51	-	expression tag
Glu -50	-	expression tag
Lys -49	-	expression tag
Thr -48	-	expression tag
Thr -47	-	expression tag
Gly -46	-	expression tag
Trp -45	-	expression tag
Arg -44	-	expression tag
Gly -43	-	expression tag
Gly -42	-	expression tag
His -41	-	expression tag
Val -40	-	expression tag
Val -39	-	expression tag
Glu -38	-	expression tag
Gly -37	-	expression tag
Leu -36	-	expression tag
Ala -35	-	expression tag
Gly -34	-	expression tag
Glu -33	-	expression tag
Leu -32	-	expression tag
Glu -31	-	expression tag
Gln -30	-	expression tag
Leu -29	-	expression tag
Arg -28	-	expression tag
Ala -27	-	expression tag
Arg -26	-	expression tag
Leu -25	-	expression tag
Glu -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
Pro -21	-	expression tag
Gln -20	-	expression tag
Gly -19	-	expression tag
Gln -18	-	expression tag
Arg -17	-	expression tag
Glu -16	-	expression tag
Pro -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag
Gly 1	-	expression tag
Gln 2	-	expression tag
Pro 3	-	expression tag
Ser 4	-	expression tag

D, A: 391 - 429 (UniProt: P14867)

PDB	External Database	Details
Ser 313	-	linker
Gln 385	-	linker
Pro 386	-	linker
Ala 387	-	linker
Arg 388	-	linker
Ala 389	-	linker
Ala 390	-	linker

E, B, C: 1 - 307 (UniProt: P28472)

PDB	External Database	Details
Met -53	-	initiating methionine
Asp -52	-	expression tag
Glu -51	-	expression tag
Lys -50	-	expression tag
Thr -49	-	expression tag
Thr -48	-	expression tag
Gly -47	-	expression tag
Trp -46	-	expression tag
Arg -45	-	expression tag
Gly -44	-	expression tag
Gly -43	-	expression tag
His -42	-	expression tag
Val -41	-	expression tag
Val -40	-	expression tag
Glu -39	-	expression tag
Gly -38	-	expression tag
Leu -37	-	expression tag
Ala -36	-	expression tag
Gly -35	-	expression tag
Glu -34	-	expression tag
Leu -33	-	expression tag
Glu -32	-	expression tag
Gln -31	-	expression tag
Leu -30	-	expression tag
Arg -29	-	expression tag
Ala -28	-	expression tag
Arg -27	-	expression tag
Leu -26	-	expression tag
Glu -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
Pro -22	-	expression tag
Gln -21	-	expression tag
Gly -20	-	expression tag
Gln -19	-	expression tag
Arg -18	-	expression tag
Glu -17	-	expression tag
Pro -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Ile -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Thr -1	-	expression tag
Gly 0	-	expression tag

E, B, C: 422 - 448 (UniProt: P28472)

PDB	External Database	Details
Ser 308	-	linker
Gln 309	-	linker
Pro 310	-	linker
Ala 311	-	linker
Arg 312	-	linker
Ala 313	-	linker
Ala 314	-	linker

F: 2 - 13 (PDB: 9FFM)
F: 14 - 234 (UniProt: B5Z8H1)
F: 235 - 403 (UniProt: B5Z8H1)
F: 404 - 523 (PDB: 9FFM)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	287349.7
Branched	Number of molecules	8
	Total formula weight	5827.3
Non-Polymers*	Number of molecules	3
	Total formula weight	426.8
All*	Total formula weight	293603.8

*Water molecules are not included.