9FFJ
Artificial metalloenzyme with a nickel-based 1,10-phenanthroline cofactor and streptavidin N49M-S112V mutant
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Streptavidin | polymer | 159 | 16598.2 | 1 | UniProt (P22629) | Streptomyces avidinii | |
| 2 | B, F (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 3 | C (A) | tetraaqua{N-methyl-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-[(1,10-phenanthrolin-4-yl-kappa~2~N~1~,N~10~)methyl]pentanamide}nickel(2+) | non-polymer | 580.3 | 1 | Chemie (A1ICD) | |||
| 4 | D, E (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) PubChem (8117) | |||
| 5 | G, H (A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 6 | I (A) | AMMONIUM ION | non-polymer | 18.0 | 1 | Chemie (NH4) PubChem (223) | |||
| 7 | J (A) | water | water | 18.0 | 36 | Chemie (HOH) |
Sequence modifications
A: 15 - 159 (UniProt: P22629)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ala 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Met 4 | - | expression tag |
| Thr 5 | - | expression tag |
| Gly 6 | - | expression tag |
| Gly 7 | - | expression tag |
| Gln 8 | - | expression tag |
| Gln 9 | - | expression tag |
| Met 10 | - | expression tag |
| Gly 11 | - | expression tag |
| Arg 12 | - | expression tag |
| Asp 13 | - | expression tag |
| Gln 14 | - | expression tag |
| Met 49 | Asn 73 | engineered mutation |
| Val 112 | Ser 136 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 16598.2 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1126.9 | |
| All* | Total formula weight | 17725.0 |
