9FF2
Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the long-lived symmetric desensitised state (C1)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E (A, B, C, D, E) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 395 | 45643.2 | 5 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit beta-3 |
| 2 | F, H, J, L, N (a, b, c, d, e) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 5 | In PDB GlyTouCan (G22768VO) | |||
| 3 | G, I, K, M, O (F, G, H, I, J) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 5 | In PDB GlyTouCan (G42666HT) | |||
| 4 | AA, HA, MA, P, SA (B, C, D, A, E) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) | |||
| 5 | CA, IA, NA, Q, V (C, D, E, A, B) | HISTAMINE | non-polymer | 111.1 | 5 | Chemie (HSM) | |||
| 6 | DA, EA, FA, JA, KA... (C, D, E, A, B) | DECANE | non-polymer | 142.3 | 15 | Chemie (D10) | |||
| 7 | BA, GA, LA, RA, U (B, C, D, E, A) | CHLORIDE ION | non-polymer | 35.5 | 5 | Chemie (CL) |
Sequence modifications
A, B, C, D, E: 1 - 307 (UniProt: P28472)
A, B, C, D, E: 422 - 448 (UniProt: P28472)
| PDB | External Database | Details |
|---|---|---|
| Met -53 | - | initiating methionine |
| Asp -52 | - | expression tag |
| Glu -51 | - | expression tag |
| Lys -50 | - | expression tag |
| Thr -49 | - | expression tag |
| Thr -48 | - | expression tag |
| Gly -47 | - | expression tag |
| Trp -46 | - | expression tag |
| Arg -45 | - | expression tag |
| Gly -44 | - | expression tag |
| Gly -43 | - | expression tag |
| His -42 | - | expression tag |
| Val -41 | - | expression tag |
| Val -40 | - | expression tag |
| Glu -39 | - | expression tag |
| Gly -38 | - | expression tag |
| Leu -37 | - | expression tag |
| Ala -36 | - | expression tag |
| Gly -35 | - | expression tag |
| Glu -34 | - | expression tag |
| Leu -33 | - | expression tag |
| Glu -32 | - | expression tag |
| Gln -31 | - | expression tag |
| Leu -30 | - | expression tag |
| Arg -29 | - | expression tag |
| Ala -28 | - | expression tag |
| Arg -27 | - | expression tag |
| Leu -26 | - | expression tag |
| Glu -25 | - | expression tag |
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| Pro -22 | - | expression tag |
| Gln -21 | - | expression tag |
| Gly -20 | - | expression tag |
| Gln -19 | - | expression tag |
| Arg -18 | - | expression tag |
| Glu -17 | - | expression tag |
| Pro -16 | - | expression tag |
| Asp -15 | - | expression tag |
| Tyr -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Ile -12 | - | expression tag |
| Pro -11 | - | expression tag |
| Thr -10 | - | expression tag |
| Thr -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Asn -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Thr -1 | - | expression tag |
| Gly 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 308 | - | linker |
| Gln 309 | - | linker |
| Pro 310 | - | linker |
| Ala 311 | - | linker |
| Arg 312 | - | linker |
| Ala 313 | - | linker |
| Ala 314 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 228216.2 | |
| Branched | Number of molecules | 10 |
| Total formula weight | 6676.1 | |
| Non-Polymers* | Number of molecules | 30 |
| Total formula weight | 3973.3 | |
| All* | Total formula weight | 238865.6 |
