9FEV

Cryo-EM structure of the beta3 homomeric GABA(A) receptor in the long-lived resting state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Gamma-aminobutyric acid receptor subunit beta-3	polymer	395	45643.2	5	UniProt (P28472) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit beta-3
2	F, H, J, L, N (a, b, c, d, e)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	5	In PDB GlyTouCan (G22768VO)
3	G, I, K, M, O (F, G, H, I, J)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	5	In PDB GlyTouCan (G42666HT)
4	P, Q, R, S, T (A, B, C, D, E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	5	Chemie (NAG)

Sequence modifications

A, B, C, D, E: 1 - 307 (UniProt: P28472)

PDB	External Database	Details
Met -53	-	initiating methionine
Asp -52	-	expression tag
Glu -51	-	expression tag
Lys -50	-	expression tag
Thr -49	-	expression tag
Thr -48	-	expression tag
Gly -47	-	expression tag
Trp -46	-	expression tag
Arg -45	-	expression tag
Gly -44	-	expression tag
Gly -43	-	expression tag
His -42	-	expression tag
Val -41	-	expression tag
Val -40	-	expression tag
Glu -39	-	expression tag
Gly -38	-	expression tag
Leu -37	-	expression tag
Ala -36	-	expression tag
Gly -35	-	expression tag
Glu -34	-	expression tag
Leu -33	-	expression tag
Glu -32	-	expression tag
Gln -31	-	expression tag
Leu -30	-	expression tag
Arg -29	-	expression tag
Ala -28	-	expression tag
Arg -27	-	expression tag
Leu -26	-	expression tag
Glu -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
Pro -22	-	expression tag
Gln -21	-	expression tag
Gly -20	-	expression tag
Gln -19	-	expression tag
Arg -18	-	expression tag
Glu -17	-	expression tag
Pro -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Ile -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Thr -1	-	expression tag
Gly 0	-	expression tag

A, B, C, D, E: 422 - 448 (UniProt: P28472)

PDB	External Database	Details
Ser 308	-	linker
Gln 309	-	linker
Pro 310	-	linker
Ala 311	-	linker
Arg 312	-	linker
Ala 313	-	linker
Ala 314	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	228216.2
Branched	Number of molecules	10
	Total formula weight	6676.1
Non-Polymers*	Number of molecules	5
	Total formula weight	1106.0
All*	Total formula weight	235998.4

*Water molecules are not included.