9FE3

Crystallographic structure of AcrB V612W

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (D)	DARPIN	polymer	169	18317.6	1		synthetic construct
2	B (A)	Multidrug efflux pump subunit AcrB	polymer	1057	114823.4	1	UniProt (P31224) Pfam (PF00873)	Escherichia coli K-12	AcrAB-TolC multidrug efflux pump subunit AcrB,Acridine resistance protein B
3	C (A)	water	water		18.0	6	Chemie (HOH)

Sequence modifications

A: 1 - 1049 (UniProt: P31224)

PDB	External Database	Details
Trp 612	Val 612	engineered mutation
Leu 1050	-	expression tag
Glu 1051	-	expression tag
His 1052	-	expression tag
His 1053	-	expression tag
His 1054	-	expression tag
His 1055	-	expression tag
His 1056	-	expression tag
His 1057	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	133140.9
All*	Total formula weight	133140.9

*Water molecules are not included.