9FDJ
Crystal structure of the NuoEF variant R66G (NuoF) from Aquifex aeolicus bound to NADH under anoxic conditions (short soak)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | NADH-quinone oxidoreductase subunit E | polymer | 160 | 18573.6 | 2 | UniProt (O66842) Pfam (PF01257) | Aquifex aeolicus VF5 | NADH dehydrogenase I subunit E,NDH-1 subunit E |
| 2 | B, D (B, D) | NADH-quinone oxidoreductase subunit F | polymer | 434 | 48423.2 | 2 | UniProt (O66841) Pfam (PF01512) Pfam (PF10531) Pfam (PF10589) | Aquifex aeolicus VF5 | |
| 3 | E, S (A, C) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 4 | BA, CA, F, G, H... (D, A, B, C) | SULFATE ION | non-polymer | 96.1 | 8 | Chemie (SO4) | |||
| 5 | I, O, V, W (A, B, C) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) PubChem (312) | |||
| 6 | J, X (B, D) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
| 7 | K, Y (B, D) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 2 | Chemie (FMN) PubChem (710) PubChem (8965) PubChem (23585816) PubChem (643976) PubChem (51435500) PubChem (51435503) PubChem (5702760) PubChem (7048779) PubChem (7048781) PubChem (15559251) | |||
| 8 | L, Z (B, D) | 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE | non-polymer | 665.4 | 2 | Chemie (NAI) PubChem (16219774) PubChem (928) PubChem (44119956) PubChem (44415182) PubChem (439153) PubChem (11250888) PubChem (12309341) PubChem (94865921) PubChem (94865923) PubChem (124934832) PubChem (126455899) PubChem (134693949) PubChem (12309339) PubChem (124432988) PubChem (12309347) PubChem (124934831) PubChem (124934833) PubChem (129607000) PubChem (129628338) PubChem (140875346) PubChem (162951601) PubChem (162951600) PubChem (164575491) PubChem (146167236) PubChem (169545638) PubChem (170905224) PubChem (174316169) PubChem (162951602) PubChem (174084875) | |||
| 9 | AA, M (D, B) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) PubChem (753) | |||
| 10 | DA, EA, FA, GA, HA... (D, B) | SODIUM ION | non-polymer | 23.0 | 8 | Chemie (NA) PubChem (923) | |||
| 11 | IA, JA, KA, LA (A, B, C, D) | water | water | 18.0 | 882 | Chemie (HOH) |
Sequence modifications
B, D: 1 - 426 (UniProt: O66841)
| PDB | External Database | Details |
|---|---|---|
| Gly 66 | Arg 66 | engineered mutation |
| Ala 427 | - | expression tag |
| Gly 428 | - | expression tag |
| His 429 | - | expression tag |
| His 430 | - | expression tag |
| His 431 | - | expression tag |
| His 432 | - | expression tag |
| His 433 | - | expression tag |
| His 434 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 133993.6 | |
| Non-Polymers* | Number of molecules | 30 |
| Total formula weight | 4576.9 | |
| All* | Total formula weight | 138570.5 |
