9FAW
CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, HEPES and Megabody25, in a desensitised state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (B, E, A, D) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 443 | 51116.7 | 4 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit beta-3,GABAAR subunit beta-3 |
| 2 | E (C) | Isoform 2 of Gamma-aminobutyric acid receptor subunit gamma-2 | polymer | 409 | 48324.9 | 1 | UniProt (P18507) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit gamma-2,GABAAR subunit gamma-2 |
| 3 | F (H) | Neuroligin-2 | polymer | 33 | 3614.1 | 1 | UniProt (Q8NFZ4) | Homo sapiens (human) | |
| 4 | G (L) | LHFPL tetraspan subfamily member 4 protein | polymer | 187 | 20503.2 | 1 | UniProt (Q7Z7J7) Pfam (PF10242) | Homo sapiens (human) | GABAA receptor regulatory Lhfpl4,Lipoma HMGIC fusion partner-like 4 protein |
| 5 | H, I, J (K, O, F) | Megabody25 | polymer | 522 | 56300.6 | 3 | Lama glama | ||
| 6 | K, M, O, Q (G, J, N, Q) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 4 | In PDB GlyTouCan (G15407YE) | |||
| 7 | L (I) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G55220VL) | |||
| 8 | N, P (M, P) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 2 | In PDB GlyTouCan (G09724ZC) | |||
| 9 | R (R) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 10 | S (S) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 11 | T (B) | HEXANE | non-polymer | 86.2 | 1 | Chemie (HEX) | |||
| 12 | U, W, Y (B, E, A) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 3 | Chemie (EPE) | |||
| 13 | V (B) | (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate | non-polymer | 749.0 | 1 | Chemie (PGW) | |||
| 14 | X, Z (E, A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 15 | AA (C) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) |
Sequence modifications
C: 21 - 428 (UniProt: P18507)
| PDB | External Database | Details |
|---|---|---|
| Gly 429 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 445811.1 | |
| Branched | Number of molecules | 9 |
| Total formula weight | 7062.4 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 2379.2 | |
| All* | Total formula weight | 455252.7 |
