9FAW

CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, HEPES and Megabody25, in a desensitised state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (B, E, A, D)	Gamma-aminobutyric acid receptor subunit beta-3	polymer	443	51116.7	4	UniProt (P28472) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit beta-3,GABAAR subunit beta-3
2	E (C)	Isoform 2 of Gamma-aminobutyric acid receptor subunit gamma-2	polymer	409	48324.9	1	UniProt (P18507) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit gamma-2,GABAAR subunit gamma-2
3	F (H)	Neuroligin-2	polymer	33	3614.1	1	UniProt (Q8NFZ4)	Homo sapiens (human)
4	G (L)	LHFPL tetraspan subfamily member 4 protein	polymer	187	20503.2	1	UniProt (Q7Z7J7) Pfam (PF10242)	Homo sapiens (human)	GABAA receptor regulatory Lhfpl4,Lipoma HMGIC fusion partner-like 4 protein
5	H, I, J (K, O, F)	Megabody25	polymer	522	56300.6	3		Lama glama
6	K, M, O, Q (G, J, N, Q)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	4	In PDB GlyTouCan (G15407YE)
7	L (I)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	1	In PDB GlyTouCan (G55220VL)
8	N, P (M, P)	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	2	In PDB GlyTouCan (G09724ZC)
9	R (R)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
10	S (S)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
11	T (B)	HEXANE	non-polymer		86.2	1	Chemie (HEX) PubChem (8058)
12	U, W, Y (B, E, A)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	3	Chemie (EPE) PubChem (23831)
13	V (B)	(1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate	non-polymer		749.0	1	Chemie (PGW)
14	X, Z (E, A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
15	AA (C)	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR) PubChem (16219173) PubChem (16758052) PubChem (40477506) PubChem (40557614) PubChem (42579431) PubChem (42579432) PubChem (45044259) PubChem (49853509) PubChem (50990013) PubChem (176980071) PubChem (18640088) PubChem (52934537) PubChem (1715243) PubChem (1715244) PubChem (23423981) PubChem (44654663) PubChem (46937002) PubChem (56621683) PubChem (59274407) PubChem (68311425) PubChem (68311398) PubChem (143173313) PubChem (177156123) PubChem (5997) PubChem (57915018) PubChem (57939063) PubChem (58428240) PubChem (58445498) PubChem (58663286) PubChem (1551269) PubChem (90070115) PubChem (59450329) PubChem (59628412) PubChem (117965027) PubChem (6432564) PubChem (59965215) PubChem (71234641) PubChem (59993755) PubChem (60001463) PubChem (66634651) PubChem (6710666) PubChem (124146240) PubChem (7059399) PubChem (124897607) PubChem (124896959) PubChem (10861996) PubChem (68055877) PubChem (68166463) PubChem (68315613) PubChem (132837164) PubChem (70795838) PubChem (70797612) PubChem (70954610) PubChem (70957185) PubChem (71463909) PubChem (11834483) PubChem (11888915) PubChem (11888914) PubChem (304) PubChem (138967089) PubChem (139566851) PubChem (89951918) PubChem (90224410) PubChem (15939944) PubChem (91298236) PubChem (191463) PubChem (368063) PubChem (369319) PubChem (638019) PubChem (101624109) PubChem (1549081) PubChem (1553803) PubChem (171828367) PubChem (173516975) PubChem (124225449) PubChem (124429284) PubChem (5283629) PubChem (5284644) PubChem (5315842) PubChem (124909223) PubChem (124909224) PubChem (124909225) PubChem (6419195) PubChem (6994560) PubChem (130459183) PubChem (7055705) PubChem (7055706) PubChem (7059398) PubChem (7059400) PubChem (7059635) PubChem (7103033) PubChem (7182477) PubChem (7182478) PubChem (131992107) PubChem (9909206) PubChem (10045721) PubChem (71168115) PubChem (10134774) PubChem (10293553) PubChem (71173031) PubChem (11003580) PubChem (11025495) PubChem (11058155) PubChem (11463329) PubChem (11868624) PubChem (11868625) PubChem (11869366) PubChem (11869367) PubChem (11869368) PubChem (11869369) PubChem (12120344) PubChem (12156156) PubChem (139568882) PubChem (12303135) PubChem (12303148) PubChem (12303156) PubChem (12303159) PubChem (12303160) PubChem (142983773) PubChem (144054483) PubChem (144209461) PubChem (144331236) PubChem (144808354) PubChem (145053568) PubChem (145157652) PubChem (145215073) PubChem (145230478) PubChem (146320991) PubChem (147857230) PubChem (154993519) PubChem (158603858) PubChem (162906452) PubChem (162906453) PubChem (162906454) PubChem (162906455) PubChem (162906456) PubChem (162906457) PubChem (163255768) PubChem (163389210) PubChem (164665303) PubChem (166553771) PubChem (166712004) PubChem (170116175) PubChem (171764280) PubChem (171817101) PubChem (171817104) PubChem (174285573)

Sequence modifications

C: 21 - 428 (UniProt: P18507)

PDB	External Database	Details
Gly 429	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	445811.1
Branched	Number of molecules	9
	Total formula weight	7062.4
Non-Polymers*	Number of molecules	8
	Total formula weight	2379.2
All*	Total formula weight	455252.7

*Water molecules are not included.