9F9P

CryoEM structure of recombinant Trypanosoma cruzi apo proteasome 20S subunit

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, O (A, O)	Proteasome subunit alpha type	polymer	268	29876.9	2	UniProt (A0A2V2W7U6) Pfam (PF10584) Pfam (PF00227)	Trypanosoma cruzi
2	B, P (B, P)	Proteasome subunit alpha type	polymer	231	25080.3	2	UniProt (A0A2V2V7B1) Pfam (PF10584) Pfam (PF00227) UniProt (by SIFTS) (A0A7J6YDC7)	Trypanosoma cruzi
3	C, Q (C, Q)	Proteasome subunit alpha type	polymer	286	31997.1	2	UniProt (A0A2V2VJR6) Pfam (PF10584) Pfam (PF00227)	Trypanosoma cruzi
4	D, R (D, R)	Proteasome subunit alpha type	polymer	247	28109.9	2	UniProt (A0A2V2WZ04) Pfam (PF10584) Pfam (PF00227) UniProt (by SIFTS) (V5BW71)	Trypanosoma cruzi
5	E, S (E, S)	Proteasome subunit alpha type	polymer	245	27163.8	2	UniProt (A0A2V2V560) Pfam (PF00227) Pfam (PF10584) UniProt (by SIFTS) (V5B1X4)	Trypanosoma cruzi
6	F, T (F, T)	Proteasome subunit alpha type	polymer	265	29400.3	2	UniProt (Q3ZMB6) Pfam (PF10584) Pfam (PF00227) UniProt (by SIFTS) (A0A7J6YFR6)	Trypanosoma cruzi
7	G, U (G, U)	Putative proteasome alpha 7 subunit	polymer	237	25643.1	2	UniProt (A0A2V2VRE2) Pfam (PF10584) Pfam (PF00227) UniProt (by SIFTS) (V5AWB3)	Trypanosoma cruzi
8	H, V (H, V)	Proteasome subunit beta	polymer	284	30778.0	2	UniProt (A0A2V2UU31) Pfam (PF00227) UniProt (by SIFTS) (V5BHX1)	Trypanosoma cruzi
9	I, W (I, W)	Proteasome subunit beta	polymer	292	32086.1	2	UniProt (V5BCU3) Pfam (PF00227)	Trypanosoma cruzi
10	J, X (J, X)	Putative proteasome beta 3 subunit	polymer	205	22424.8	2	UniProt (A0A2V2UWV1) Pfam (PF00227) UniProt (by SIFTS) (V5BGL9)	Trypanosoma cruzi
11	K, Y (K, Y)	Proteasome subunit beta	polymer	206	22745.2	2	UniProt (A0A2V2VNZ4) Pfam (PF00227) UniProt (by SIFTS) (V5C017)	Trypanosoma cruzi
12	L, Z (L, Z)	Proteasome subunit beta	polymer	311	34977.4	2	UniProt (A0A2V2V5A7) Pfam (PF00227) UniProt (by SIFTS) (V5BU39)	Trypanosoma cruzi
13	AA, M (M, a)	Proteasome subunit beta	polymer	246	27832.5	2	UniProt (A0A2V2VW62) Pfam (PF00227)	Trypanosoma cruzi
14	BA, N (N, b)	Proteasome subunit beta	polymer	218	24222.8	2	UniProt (A0A2V2VV98) Pfam (PF00227)	Trypanosoma cruzi
15	AB, BB, CA, CB, DA... (Y, Z, A, a, B...)	water	water		18.0	1074	Chemie (HOH)

Sequence modifications

A, O: 1 - 250 (UniProt: A0A2V2W7U6)

PDB	External Database	Details
Ala 251	-	expression tag
Glu 252	-	expression tag
Asn 253	-	expression tag
Leu 254	-	expression tag
Tyr 255	-	expression tag
Phe 256	-	expression tag
Gln 257	-	expression tag
Ser 258	-	expression tag
His 259	-	expression tag
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag
His 266	-	expression tag
His 267	-	expression tag
His 268	-	expression tag

D, R: 1 - 247 (UniProt: A0A2V2WZ04)

PDB	External Database	Details
Arg 223	His 223	conflict

M, a: 1 - 246 (UniProt: A0A2V2VW62)

PDB	External Database	Details
Asn 245	Lys 245	conflict

Contents of the asymmetric unit

Polymers	Number of chains	28
	Total formula weight	784676.4
All*	Total formula weight	784676.4

*Water molecules are not included.