9F47

crystal structure of [FeFe]-hydrogenase CbA5H from Clostridium beijerinckii in Hinact state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	[FeFe]-hydrogenase	polymer	674	75001.9	2	UniProt (A0A1I9RYV3) Pfam (PF10531) Pfam (PF14697) Pfam (PF02906) Pfam (PF02256)	Clostridium beijerinckii
2	C, N (A, B)	dicarbonyl[bis(cyanide-kappaC)]-mu-(iminodimethanethiolatato-1kappaS:2kappaS)-mu-(oxomethylidene)diiron(2+)	non-polymer		355.0	2	Chemie (402)
3	D, E, F, O, P... (A, B)	IRON/SULFUR CLUSTER	non-polymer		351.6	6	Chemie (SF4)
4	G, R (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	H, I, J, K, L... (A, B)	CHLORIDE ION	non-polymer		35.5	10	Chemie (CL)
6	W, X (A, B)	water	water		18.0	32	Chemie (HOH)

Sequence modifications

A, B: 1 - 644 (UniProt: A0A1I9RYV3)

PDB	External Database	Details
Asp 645	-	expression tag
Ile 646	-	expression tag
Trp 647	-	expression tag
Ser 648	-	expression tag
Val 649	-	expression tag
Gly 650	-	expression tag
Val 651	-	expression tag
Lys 652	-	expression tag
Leu 653	-	expression tag
Phe 654	-	expression tag
Gly 655	-	expression tag
Gly 656	-	expression tag
Gly 657	-	expression tag
Ser 658	-	expression tag
Gly 659	-	expression tag
Gly 660	-	expression tag
Gly 661	-	expression tag
Ser 662	-	expression tag
Gly 663	-	expression tag
Gly 664	-	expression tag
Gly 665	-	expression tag
Ser 666	-	expression tag
Trp 667	-	expression tag
Ser 668	-	expression tag
His 669	-	expression tag
Pro 670	-	expression tag
Gln 671	-	expression tag
Phe 672	-	expression tag
Glu 673	-	expression tag
Lys 674	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	150003.9
Non-Polymers*	Number of molecules	20
	Total formula weight	3305.1
All*	Total formula weight	153309.0

*Water molecules are not included.