9F2L
Cryo-EM structure of the I923V MDA5-dsRNA filament with ADP-AlF4 bound and 73-degree helical twist
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Interferon-induced helicase C domain-containing protein 1 | polymer | 1028 | 116683.1 | 1 | UniProt (Q8R5F7) Pfam (PF16739) Pfam (PF00619) Pfam (PF04851) Pfam (PF18119) Pfam (PF00271) Pfam (PF11648) | Mus musculus (house mouse) | Helicase with 2 CARD domains,Helicard,Interferon induced with helicase C domain protein 1,Melanoma differentiation-associated protein 5,MDA-5,RIG-I-like receptor 2,RLR-2 |
| 2 | B (X) | RNA (5'-R(P*CP*AP*AP*GP*CP*CP*GP*AP*GP*GP*AP*GP*AP*G)-3') | polymer | 14 | 4587.9 | 1 | Mus musculus | ||
| 3 | C (Y) | RNA (5'-R(P*CP*UP*CP*UP*CP*CP*UP*CP*GP*GP*CP*UP*UP*G)-3') | polymer | 14 | 4352.6 | 1 | Mus musculus | ||
| 4 | D (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 5 | E (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 6 | F (A) | TETRAFLUOROALUMINATE ION | non-polymer | 103.0 | 1 | Chemie (ALF) |
Sequence modifications
A: 3 - 1025 (UniProt: Q8R5F7)
| PDB | External Database | Details |
|---|---|---|
| Met -20 | - | initiating methionine |
| Gly -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Ser -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Arg -7 | - | expression tag |
| Glu -6 | - | expression tag |
| Asn -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
| His 1 | - | expression tag |
| Met 2 | - | expression tag |
| - | Asp 646 | deletion |
| - | Lys 647 | deletion |
| - | Ser 648 | deletion |
| - | Asp 649 | deletion |
| - | Asp 650 | deletion |
| - | Glu 651 | deletion |
| - | Ala 652 | deletion |
| - | Ser 653 | deletion |
| - | Ser 654 | deletion |
| - | Cys 655 | deletion |
| - | Asn 656 | deletion |
| - | Asp 657 | deletion |
| - | Gln 658 | deletion |
| - | Leu 659 | deletion |
| - | Lys 660 | deletion |
| - | Gly 661 | deletion |
| - | Asp 662 | deletion |
| - | Val 663 | deletion |
| Val 923 | Ile 923 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 125623.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 595.6 | |
| All* | Total formula weight | 126219.1 |
