9F28

Crystal structure of the heterodimeric primase from pyrococcus abyssi (deletion of the PriL-CTD domain)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA primase small subunit PriS	polymer	351	41050.0	1	UniProt (Q9V292) Pfam (PF01896)	Pyrococcus abyssi	DNA primase 41 kDa subunit,Pabp41,p41
2	B (B)	DNA primase large subunit PriL	polymer	223	26147.1	1	UniProt (Q9V291) Pfam (PF04104)	Pyrococcus abyssi	DNA primase 46 kDa subunit,Pabp46,p46
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	D, E (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	F, G (A, B)	FORMIC ACID	non-polymer		46.0	2	Chemie (FMT)
6	H, I (A, B)	water	water		18.0	611	Chemie (HOH)

Sequence modifications

A: 1 - 345 (UniProt: Q9V292)

PDB	External Database	Details
Gly -5	-	expression tag
Thr -4	-	expression tag
Gly -3	-	expression tag
Asp -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

B: 1 - 215 (UniProt: Q9V291)

PDB	External Database	Details
Asp 216	-	expression tag
Ile 217	-	expression tag
Gly 218	-	expression tag
Arg 219	-	expression tag
Pro 220	-	expression tag
Arg 221	-	expression tag
Asp 222	-	expression tag
Arg 223	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	67197.1
Non-Polymers*	Number of molecules	5
	Total formula weight	206.1
All*	Total formula weight	67403.2

*Water molecules are not included.