9F1R

Cryo-EM structure of SV2B-Hc-A1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Synaptic vesicle glycoprotein 2B	polymer	683	77515.0	1	UniProt (Q7L1I2) Pfam (PF00083) Pfam (PF07690)	Homo sapiens (human)
2	B (B)	Botulinum neurotoxin A heavy chain	polymer	443	51724.6	1	UniProt (P0DPI0) Pfam (PF07953) Pfam (PF07951) UniProt (by SIFTS) (P0DPI1)	Clostridium botulinum	HC
3	C (C)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G28454KX)
4	D (D)	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		894.8	1	In PDB GlyTouCan (G00395TQ)
5	E (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)

Sequence modifications

B: 871 - 1296 (UniProt: P0DPI0)

PDB	External Database	Details
Met 854	-	initiating methionine
Lys 855	-	expression tag
Lys 856	-	expression tag
His 857	-	expression tag
His 858	-	expression tag
His 859	-	expression tag
His 860	-	expression tag
His 861	-	expression tag
His 862	-	expression tag
Gly 863	-	expression tag
Ser 864	-	expression tag
Leu 865	-	expression tag
Val 866	-	expression tag
Pro 867	-	expression tag
Arg 868	-	expression tag
Gly 869	-	expression tag
Ser 870	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	129239.6
Branched	Number of molecules	2
	Total formula weight	1465.4
Non-Polymers*	Number of molecules	1
	Total formula weight	221.2
All*	Total formula weight	130926.2

*Water molecules are not included.