9F0M
Crystal structure of Ta_Cel5A E133Q Y200F variant in complex with cellopentaose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | cellulase | polymer | 306 | 33843.5 | 2 | UniProt (Q8TG26) Pfam (PF00150) | Thermoascus aurantiacus ATCC 26904 | |
| 2 | C, D (C, D) | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 828.7 | 2 | In PDB GlyTouCan (G91683DU) | |||
| 3 | E, F, G, H (A, B) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 4 | I, J (B) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 5 | K, L (A, B) | water | water | 18.0 | 944 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 305 (UniProt: Q8TG26)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Gln 133 | Glu 163 | engineered mutation |
| Phe 200 | Tyr 230 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 67687.1 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1657.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 560.5 | |
| All* | Total formula weight | 69905.0 |
