9EZD
BsmI (Bottom Nicking mutant) crystallized with Mg2+ and cognate dsDNA (Post-reactive complex)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | BsmI | polymer | 676 | 78190.2 | 1 | UniProt (Q8RLN4) | Geobacillus stearothermophilus | |
| 2 | B (E) | DNA (Bottom strand - 5'-part) | polymer | 8 | 2337.6 | 1 | Geobacillus stearothermophilus | ||
| 3 | C (F) | DNA (Top strand) | polymer | 13 | 4049.7 | 1 | Geobacillus stearothermophilus | ||
| 4 | D (G) | DNA (Bottom strand - 3'-part) | polymer | 5 | 1511.0 | 1 | Geobacillus stearothermophilus | ||
| 5 | E (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 6 | F (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 7 | G (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 8 | H, I, J (A, F, G) | water | water | 18.0 | 51 | Chemie (HOH) |
Sequence modifications
A: 1 - 676 (UniProt: Q8RLN4)
| PDB | External Database | Details |
|---|---|---|
| Asp 507 | Arg 507 | engineered mutation |
| Val 509 | Gly 509 | engineered mutation |
| Val 546 | Glu 546 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 86088.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 255.0 | |
| All* | Total formula weight | 86343.5 |
