9EZ7
BsmI (Nicking top mutant) crystallized with Ca2+ and cognate dsDNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | BsmI | polymer | 669 | 77504.4 | 1 | UniProt (Q8RLN4) | Geobacillus stearothermophilus | |
| 2 | B (E) | DNA (Bottom strand) | polymer | 13 | 3893.5 | 1 | Geobacillus stearothermophilus | ||
| 3 | C (F) | DNA (Top strand) | polymer | 13 | 4049.7 | 1 | Geobacillus stearothermophilus | ||
| 4 | D (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 5 | E (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 6 | F, G (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 7 | H, I, J (A, E, F) | water | water | 18.0 | 476 | Chemie (HOH) |
Sequence modifications
A: 1 - 676 (UniProt: Q8RLN4)
| PDB | External Database | Details |
|---|---|---|
| Val 109 | Glu 109 | engineered mutation |
| - | Cys 488 | deletion |
| - | Phe 489 | deletion |
| - | Ser 490 | deletion |
| - | Gly 491 | deletion |
| - | Met 492 | deletion |
| - | Cys 493 | deletion |
| - | Gly 582 | deletion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 85447.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 310.8 | |
| All* | Total formula weight | 85758.5 |
