9EX9
Cryo EM map and model of the vaccinia minimal RNA polymerase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase 147 kDa polypeptide | polymer | 1286 | 146995.7 | 1 | UniProt (P20504) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Vaccinia virus | |
| 2 | B (B) | DNA-directed RNA polymerase 133 kDa polypeptide | polymer | 1164 | 133526.9 | 1 | UniProt (P68694) Pfam (PF04560) Pfam (PF12415) Pfam (PF04565) Pfam (PF04567) Pfam (PF00562) | Vaccinia virus | |
| 3 | C (C) | DNA-directed RNA polymerase 35 kDa subunit | polymer | 305 | 35430.7 | 1 | UniProt (P21087) Pfam (PF03396) | Vaccinia virus | |
| 4 | D (E) | DNA-directed RNA polymerase 22 kDa subunit | polymer | 186 | 21391.8 | 1 | UniProt (P68608) Pfam (PF05273) | Vaccinia virus | |
| 5 | E (F) | DNA-directed RNA polymerase 19 kDa subunit | polymer | 164 | 19020.1 | 1 | UniProt (P68610) Pfam (PF05320) | Vaccinia virus | |
| 6 | F (G) | DNA-directed RNA polymerase 18 kDa subunit | polymer | 161 | 17917.2 | 1 | UniProt (P21034) Pfam (PF03293) | Vaccinia virus | |
| 7 | G (J) | DNA-directed RNA polymerase 7 kDa subunit | polymer | 63 | 7299.7 | 1 | UniProt (P68315) Pfam (PF05864) | Vaccinia virus | |
| 8 | H (S) | DNA-directed RNA polymerase 30 kDa polypeptide | polymer | 259 | 30074.3 | 1 | UniProt (P21082) Pfam (PF12410) Pfam (PF01096) | Vaccinia virus | |
| 9 | I (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | J, K, L, M (A, B, J) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) |
Sequence modifications
A: 1 - 1286 (UniProt: P20504)
B: 1 - 1164 (UniProt: P68694)
C: 1 - 305 (UniProt: P21087)
E: 1 - 185 (UniProt: P68608)
| PDB | External Database | Details |
|---|---|---|
| Thr 258 | Ser 258 | variant |
| Glu 489 | Lys 489 | variant |
| Lys 1015 | Arg 1015 | variant |
| PDB | External Database | Details |
|---|---|---|
| Asn 6 | Asp 6 | variant |
| Phe 343 | Tyr 343 | variant |
| PDB | External Database | Details |
|---|---|---|
| Asn 236 | Asp 236 | variant |
| PDB | External Database | Details |
|---|---|---|
| Ace 0 | - | acetylation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 411656.3 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 285.9 | |
| All* | Total formula weight | 411942.2 |
