9EWR

Crystal structure of an inverse charged cutinase mutant from Saccharopolyspora flava (611)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	cutinase	polymer	269	29670.7	2	UniProt (A0A1I6NU60) Pfam (PF01738)	Saccharopolyspora flava
2	C, D (A, B)	water	water		18.0	462	Chemie (HOH)

Sequence modifications

A, B: 2 - 261 (UniProt: A0A1I6NU60)

PDB	External Database	Details
Met 1	-	initiating methionine
Lys 6	Asp 38	engineered mutation
Lys 11	Asp 43	engineered mutation
Lys 19	Ala 51	engineered mutation
Asn 27	Asp 59	engineered mutation
Arg 28	Glu 60	engineered mutation
Arg 29	Glu 61	engineered mutation
Lys 35	Ser 67	engineered mutation
Lys 37	Ser 69	engineered mutation
Asn 49	Asp 81	engineered mutation
Lys 109	Asp 141	engineered mutation
Lys 114	Asp 146	engineered mutation
Lys 116	Asp 148	engineered mutation
Lys 147	Ala 179	engineered mutation
Arg 164	Ser 196	engineered mutation
Arg 167	Gln 199	engineered mutation
Glu 188	Lys 220	engineered mutation
Lys 192	Glu 224	engineered mutation
Lys 197	Asp 229	engineered mutation
Arg 240	Gln 272	engineered mutation
Arg 247	Glu 279	engineered mutation
Asn 249	Asp 281	engineered mutation
Lys 250	Asp 282	engineered mutation
Arg 256	Gln 288	engineered mutation
Lys 262	-	expression tag
Glu 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag
His 266	-	expression tag
His 267	-	expression tag
His 268	-	expression tag
His 269	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	59341.3
All*	Total formula weight	59341.3

*Water molecules are not included.