9EVV
His579Leu variant of L-arabinonate dehydratase co-crystallized with 2-oxobutyrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | L-arabinonate dehydratase | polymer | 588 | 63833.9 | 4 | UniProt (B5ZZ34) Pfam (PF00920) | Rhizobium leguminosarum bv. trifolii | ArDHT,D-fuconate dehydratase,Galactonate dehydratase,L-arabonate dehydratase |
| 2 | E, G, I, K (A, B, C, D) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 3 | F, H, J, L (A, B, C, D) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 4 | Chemie (FES) | |||
| 4 | M (D) | 2-KETOBUTYRIC ACID | non-polymer | 102.1 | 1 | Chemie (2KT) | |||
| 5 | N, O, P, Q (A, B, C, D) | water | water | 18.0 | 431 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 2 - 579 (UniProt: B5ZZ34)
| PDB | External Database | Details |
|---|---|---|
| Met -8 | - | initiating methionine |
| Asp -7 | - | expression tag |
| Trp -6 | - | expression tag |
| Ser -5 | - | expression tag |
| His -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Phe -1 | - | expression tag |
| Glu 0 | - | expression tag |
| Lys 1 | - | expression tag |
| Leu 579 | His 579 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 255335.8 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 902.6 | |
| All* | Total formula weight | 256238.4 |
