9EQL
Hydrogenase-1 Ni-B state poised at +300mV
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (S, T) | Hydrogenase-1 small chain | polymer | 279 | 30980.4 | 2 | UniProt (P69739) Pfam (PF01058) Pfam (PF14720) | Escherichia coli | HYD1,Membrane-bound hydrogenase 1 small subunit,NiFe hydrogenase |
| 2 | B, D (L, M) | Hydrogenase-1 large chain | polymer | 582 | 64751.4 | 2 | UniProt (P0ACD8) Pfam (PF00374) | Escherichia coli | HYD1,Membrane-bound hydrogenase 1 large subunit,NiFe hydrogenase |
| 3 | E, N (S, T) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
| 4 | F, O (S, T) | FE3-S4 CLUSTER | non-polymer | 295.8 | 2 | Chemie (F3S) | |||
| 5 | G, P (S, T) | FE4-S3 CLUSTER | non-polymer | 319.6 | 2 | Chemie (SF3) | |||
| 6 | H, I, Q, R (S, T) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 7 | J (L) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 8 | K, S (L, M) | CARBONMONOXIDE-(DICYANO) IRON | non-polymer | 135.9 | 2 | Chemie (FCO) | |||
| 9 | L, T (L, M) | NICKEL (II) ION | non-polymer | 58.7 | 2 | Chemie (NI) | |||
| 10 | M, U (L, M) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 11 | V, W, X, Y (S, L, T, M) | water | water | 18.0 | 1271 | Chemie (HOH) |
Sequence modifications
S, T: 1 - 271 (UniProt: P69739)
| PDB | External Database | Details |
|---|---|---|
| Arg 272 | - | expression tag |
| Ser 273 | - | expression tag |
| His 274 | - | expression tag |
| His 275 | - | expression tag |
| His 276 | - | expression tag |
| His 277 | - | expression tag |
| His 278 | - | expression tag |
| His 279 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 191463.5 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 2609.7 | |
| All* | Total formula weight | 194073.2 |
