9EQG
CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with GABA and puerarin
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | Gamma-aminobutyric acid receptor subunit alpha-1 | polymer | 464 | 52916.6 | 2 | UniProt (P14867) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit alpha-1 |
| 2 | B, E (B, E) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 473 | 54180.3 | 2 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit beta-3 |
| 3 | C (C) | Gamma-aminobutyric acid receptor subunit gamma-2 | polymer | 495 | 56922.1 | 1 | UniProt (P18507) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit gamma-2 |
| 4 | F (F) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 1 | In PDB GlyTouCan (G40702WU) | |||
| 5 | G, I, J (G, I, J) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 3 | In PDB GlyTouCan (G09724ZC) | |||
| 6 | H, K (H, K) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
| 7 | L, LA (A, D) | (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate | non-polymer | 749.0 | 2 | Chemie (PGW) | |||
| 8 | CA, EA, GA, M, N... (C, A, D, E, B) | DECANE | non-polymer | 142.3 | 11 | Chemie (D10) | |||
| 9 | BA, FA, IA, O, R... (B, C, A, E) | HEXANE | non-polymer | 86.2 | 10 | Chemie (HEX) | |||
| 10 | P, QA (A, D) | [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate | non-polymer | 1047.1 | 2 | Chemie (PT5) | |||
| 11 | JA, Q, TA, W, Y (C, A, E, B) | HEXADECANE | non-polymer | 226.4 | 5 | Chemie (R16) | |||
| 12 | DA, MA, U (C, D, A) | PALMITIC ACID | non-polymer | 256.4 | 3 | Chemie (PLM) | |||
| 13 | KA, SA, V (C, D, A) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 14 | AA, XA (B, E) | GAMMA-AMINO-BUTANOIC ACID | non-polymer | 103.1 | 2 | Chemie (ABU) | |||
| 15 | HA (C) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 16 | PA (D) | Puerarin | non-polymer | 416.4 | 1 | Chemie (A1H6W) | |||
| 17 | RA (D) | 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE | non-polymer | 535.7 | 1 | Chemie (PX2) | |||
| 18 | AB, BB, CB, DB, EB (A, B, C, D, E) | water | water | 18.0 | 258 | Chemie (HOH) |
Sequence modifications
A, D: 1 - 429 (UniProt: P14867)
C: -38 - 436 (UniProt: P18507)
| PDB | External Database | Details |
|---|---|---|
| Met -34 | - | initiating methionine |
| Lys -33 | - | expression tag |
| Lys -32 | - | expression tag |
| Ser -31 | - | expression tag |
| Pro -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Leu -28 | - | expression tag |
| Ser -27 | - | expression tag |
| Asp -26 | - | expression tag |
| Tyr -25 | - | expression tag |
| Leu -24 | - | expression tag |
| Trp -23 | - | expression tag |
| Ala -22 | - | expression tag |
| Trp -21 | - | expression tag |
| Thr -20 | - | expression tag |
| Leu -19 | - | expression tag |
| Phe -18 | - | expression tag |
| Leu -17 | - | expression tag |
| Ser -16 | - | expression tag |
| Thr -15 | - | expression tag |
| Leu -14 | - | expression tag |
| Thr -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Arg -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Tyr -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Asp -7 | - | expression tag |
| Tyr -6 | - | expression tag |
| Lys -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Lys 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 437 | - | expression tag |
| Gly 438 | - | expression tag |
| Ser 439 | - | expression tag |
| Gly 440 | - | expression tag |
| Gly 441 | - | expression tag |
| Ser 442 | - | expression tag |
| Gly 443 | - | expression tag |
| Gly 444 | - | expression tag |
| Ser 445 | - | expression tag |
| Gly 446 | - | expression tag |
| Lys 447 | - | expression tag |
| Thr 448 | - | expression tag |
| Glu 449 | - | expression tag |
| Thr 450 | - | expression tag |
| Ser 451 | - | expression tag |
| Gln 452 | - | expression tag |
| Val 453 | - | expression tag |
| Ala 454 | - | expression tag |
| Pro 455 | - | expression tag |
| Ala 456 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 271116.0 | |
| Branched | Number of molecules | 6 |
| Total formula weight | 6113.5 | |
| Non-Polymers* | Number of molecules | 41 |
| Total formula weight | 9406.4 | |
| All* | Total formula weight | 286635.8 |
