9EMV

SARS-CoV-2 nsp10-16 methyltransferase in complex with Sangivamycin and m7GpppA (Cap0-analog)/m7GpppAm (Cap1-analog)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	2'-O-methyltransferase nsp16	polymer	304	34150.1	1	UniProt (P0DTD1) Pfam (PF06460)	Severe acute respiratory syndrome coronavirus 2	Non-structural protein 16,nsp16
2	B (B)	Non-structural protein 10	polymer	140	14860.0	1	UniProt (P0DTD1) Pfam (PF09401)	Severe acute respiratory syndrome coronavirus 2	nsp10,Growth factor-like peptide,GFL
3	C (A)	SANGIVAMYCIN	non-polymer		309.3	1	Chemie (SGV)
4	D (A)	P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE	non-polymer		787.4	1	Chemie (GTA)
5	E (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
6	F (A)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
7	G, H, I, J, K... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	8	Chemie (EDO)
8	M (A)	7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-ADENOSINE	non-polymer		801.5	1	Chemie (V9G)
9	N, O (B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10	R, S (A, B)	water	water		18.0	151	Chemie (HOH)

Sequence modifications

A: 6799 - 7096 (UniProt: P0DTD1)

PDB	External Database	Details
Glu 7097	-	expression tag
Asn 7098	-	expression tag
Leu 7099	-	expression tag
Tyr 7100	-	expression tag
Phe 7101	-	expression tag
Gln 7102	-	expression tag

B: 4254 - 4392 (UniProt: P0DTD1)

PDB	External Database	Details
Gly 4253	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	49010.1
Non-Polymers*	Number of molecules	15
	Total formula weight	3105.2
All*	Total formula weight	52115.3

*Water molecules are not included.