9EMF

KOD-H4 DNA polymerase mutant in a binary complex with DNA:DNA containing one HNA nucleotide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase	polymer	774	89976.5	1	UniProt (D0VWU9)	Thermococcus kodakarensis KOD1
2	B (P)	DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*CP*AP*CP*A)-3')	polymer	13	3930.6	1		synthetic construct
3	C (T)	DNA (5'-D(P*AP*CP*(6HT)P*GP*TP*GP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3')	polymer	16	4944.2	1		synthetic construct
4	D, E, F, G, H (A)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
5	I (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
6	J, L (A, P)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
7	K (A)	(2S)-2-hydroxy-3-(morpholin-4-yl)propane-1-sulfonic acid	non-polymer		225.3	1	Chemie (6BX)
8	M, N (A, T)	water	water		18.0	9	Chemie (HOH)

Sequence modifications

A: 1 - 774 (UniProt: D0VWU9)

PDB	External Database	Details
Gln 93	Val 93	conflict
Thr 114	Ile 114	conflict
Ala 141	Asp 141	conflict
Ala 143	Glu 143	conflict
His 147	Glu 147	conflict
Lys 383	Ser 383	conflict
Gly 429	Lys 429	conflict
Leu 445	Phe 445	conflict
Leu 485	Ala 485	conflict
Val 493	Tyr 493	conflict
His 496	Tyr 496	conflict
Met 497	Tyr 497	conflict
Phe 499	Tyr 499	conflict
Glu 500	Ala 500	conflict
Asn 501	Arg 501	conflict
Leu 521	Ile 521	conflict
Lys 584	Glu 584	conflict
Lys 664	Glu 664	conflict
Arg 726	Lys 726	conflict
Lys 735	Asn 735	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	98851.3
Non-Polymers*	Number of molecules	9
	Total formula weight	554.0
All*	Total formula weight	99405.3

*Water molecules are not included.