9EJC

Cryo-EM Structure of CXCL1-KSHV ORF74-Gi-scFv16 Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	360	39660.3	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
2	B (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
3	C (E)	viral G-protein coupled receptor	polymer	348	39601.5	1	UniProt (Q98146) Pfam (PF00001)	Human gammaherpesvirus 8	vGPCR,Protein ORF74
4	D (F)	Growth-regulated alpha protein	polymer	91	10131.8	1	UniProt (P09341) Pfam (PF00048)	Homo sapiens (human)	C-X-C motif chemokine 1,GRO-alpha(1-73),Melanoma growth stimulatory activity,MGSA,Neutrophil-activating protein 3,NAP-3
5	E (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
6	F (D)	scFv Recombinant Mouse Monoclonal Antibody (scFv16)	polymer	287	30895.5	1		Mus musculus

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Leu -11	-	expression tag
Glu -10	-	expression tag
Val -9	-	expression tag
Leu -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Gly -5	-	expression tag
Pro -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

E: 1 - 340 (UniProt: Q98146)

PDB	External Database	Details
Cys 30	Gly 30	engineered mutation
Trp 169	Leu 169	engineered mutation
Val 258	Leu 258	engineered mutation
Asp 341	-	expression tag
Tyr 342	-	expression tag
Lys 343	-	expression tag
Asp 344	-	expression tag
Asp 345	-	expression tag
Asp 346	-	expression tag
Asp 347	-	expression tag
Lys 348	-	expression tag

F: 1 - 73 (UniProt: P09341)

PDB	External Database	Details
Cys 22	Asn 56	engineered mutation
Leu 74	-	expression tag
Glu 75	-	expression tag
Val 76	-	expression tag
Leu 77	-	expression tag
Phe 78	-	expression tag
Gln 79	-	expression tag
Gly 80	-	expression tag
Pro 81	-	expression tag
His 82	-	expression tag
His 83	-	expression tag
His 84	-	expression tag
His 85	-	expression tag
His 86	-	expression tag
His 87	-	expression tag
His 88	-	expression tag
His 89	-	expression tag
His 90	-	expression tag
His 91	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	168565.3
All*	Total formula weight	168565.3

*Water molecules are not included.