9EJ8
Structure of the Lactate Monooxygenase mutant Y44N, Y152F, H290S, T181A, F184P
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | L-lactate 2-monooxygenase | polymer | 402 | 43668.1 | 1 | UniProt (P21795) | Mycolicibacterium smegmatis | LMO,Lactate monooxygenase |
| 2 | B (A) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 1 | Chemie (FMN) PubChem (710) PubChem (8965) PubChem (643976) PubChem (5702760) PubChem (7048779) PubChem (7048781) PubChem (23585816) PubChem (51435500) PubChem (51435503) PubChem (15559251) | |||
| 3 | C, E (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) PubChem (753) | |||
| 4 | D (A) | CITRIC ACID | non-polymer | 192.1 | 1 | Chemie (CIT) PubChem (311) | |||
| 5 | F, G, H (A) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 6 | I (A) | water | water | 18.0 | 521 | Chemie (HOH) |
Sequence modifications
A: 0 - 393 (UniProt: P21795)
| PDB | External Database | Details |
|---|---|---|
| Asn 44 | Tyr 45 | engineered mutation |
| Phe 152 | Tyr 153 | engineered mutation |
| Ala 181 | Thr 182 | engineered mutation |
| Pro 184 | Phe 185 | engineered mutation |
| Ser 290 | His 291 | engineered mutation |
| Leu 394 | - | expression tag |
| Glu 395 | - | expression tag |
| His 396 | - | expression tag |
| His 397 | - | expression tag |
| His 398 | - | expression tag |
| His 399 | - | expression tag |
| His 400 | - | expression tag |
| His 401 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 43668.1 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1120.8 | |
| All* | Total formula weight | 44788.9 |
