9EI4
Cryo-EM structure of Human RNA polymerase II Elongation Complex bound to the RECQL5 helicase in the presence of ADP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase II subunit RPB1 | polymer | 1970 | 217420.0 | 1 | UniProt (P24928) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) | Homo sapiens (human) | RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1 |
| 2 | B (B) | DNA-directed RNA polymerase II subunit RPB2 | polymer | 1174 | 134071.5 | 1 | UniProt (P30876) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) | Homo sapiens (human) | DNA-directed RNA polymerase II 140 kDa polypeptide,DNA-directed RNA polymerase II subunit B,RNA polymerase II subunit 2,RNA polymerase II subunit B2 |
| 3 | C (C) | DNA-directed RNA polymerase II subunit RPB3 | polymer | 275 | 31478.1 | 1 | UniProt (P19387) Pfam (PF01193) Pfam (PF01000) | Homo sapiens (human) | RNA polymerase II subunit B3,DNA-directed RNA polymerase II 33 kDa polypeptide,RPB33,DNA-directed RNA polymerase II subunit C,RPB31 |
| 4 | D (D) | DNA-directed RNA polymerase II subunit RPB4 | polymer | 142 | 16331.3 | 1 | UniProt (O15514) Pfam (PF03874) | Homo sapiens (human) | RNA polymerase II subunit B4,DNA-directed RNA polymerase II subunit D,RNA polymerase II 16 kDa subunit,RPB16 |
| 5 | E (E) | DNA-directed RNA polymerases I, II, and III subunit RPABC1 | polymer | 210 | 24644.3 | 1 | UniProt (P19388) Pfam (PF03871) Pfam (PF01191) | Homo sapiens (human) | RNA polymerases I,II,and III subunit ABC1,DNA-directed RNA polymerase II 23 kDa polypeptide,DNA-directed RNA polymerase II subunit E,RPB5 homolog,XAP4 |
| 6 | F (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 127 | 14491.0 | 1 | UniProt (P61218) Pfam (PF01192) | Homo sapiens (human) | RNA polymerases I,II,and III subunit ABC2,DNA-directed RNA polymerase II subunit F,DNA-directed RNA polymerases I,and III 14.4 kDa polypeptide,RPABC14.4,RPB14.4,RPB6 homolog,RPC15 |
| 7 | G (G) | DNA-directed RNA polymerase II subunit RPB7 | polymer | 172 | 19314.3 | 1 | UniProt (P62487) Pfam (PF03876) Pfam (PF00575) | Homo sapiens (human) | RNA polymerase II subunit B7,DNA-directed RNA polymerase II subunit G,RNA polymerase II 19 kDa subunit,RPB19 |
| 8 | H (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 150 | 17162.3 | 1 | UniProt (P52434) Pfam (PF03870) | Homo sapiens (human) | RNA polymerases I,II,and III subunit ABC3,DNA-directed RNA polymerase II subunit H,DNA-directed RNA polymerases I,and III 17.1 kDa polypeptide,RPB17,RPB8 homolog,hRPB8 |
| 9 | I (I) | DNA-directed RNA polymerase II subunit RPB9 | polymer | 125 | 14541.2 | 1 | UniProt (P36954) Pfam (PF02150) Pfam (PF01096) | Homo sapiens (human) | RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5 |
| 10 | J (J) | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 67 | 7655.1 | 1 | UniProt (P62875) Pfam (PF01194) | Homo sapiens (human) | RNA polymerases I,II,and III subunit ABC5,DNA-directed RNA polymerase III subunit L,RNA polymerase II 7.6 kDa subunit,RPB7.6,RPB10 homolog |
| 11 | K (K) | DNA-directed RNA polymerase II subunit RPB11-a | polymer | 117 | 13310.3 | 1 | UniProt (P52435) Pfam (PF13656) | Homo sapiens (human) | RPB11a,DNA-directed RNA polymerase II subunit J-1,RNA polymerase II 13.3 kDa subunit |
| 12 | L (L) | DNA-directed RNA polymerases I, II, and III subunit RPABC4 | polymer | 58 | 7018.2 | 1 | UniProt (P53803) Pfam (PF03604) | Homo sapiens (human) | RNA polymerases I,II,and III subunit ABC4,ABC10-alpha,DNA-directed RNA polymerase II subunit K,RNA polymerase II 7.0 kDa subunit,RPB7.0,RPB10alpha |
| 13 | M (N) | Non-template DNA, nucleic acid scaffold | polymer | 43 | 13305.6 | 1 | Homo sapiens | ||
| 14 | N (P) | RNA, nucleic acid scaffold | polymer | 20 | 6414.9 | 1 | Homo sapiens | ||
| 15 | O (T) | Template DNA, nucleic acid scaffold | polymer | 28 | 8515.5 | 1 | Homo sapiens | ||
| 16 | P (U) | ATP-dependent DNA helicase Q5 | polymer | 991 | 109024.9 | 1 | UniProt (O94762) Pfam (PF00270) Pfam (PF00271) Pfam (PF16124) Pfam (PF06959) Pfam (PF08236) | Homo sapiens (human) | DNA 3'-5' helicase RecQ5,DNA helicase,RecQ-like type 5,RecQ5,RecQ protein-like 5 |
| 17 | Q, R, T, U, V... (A, B, C, I, J...) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 18 | S (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 19 | Z (U) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) |
Sequence modifications
E: 1 - 210 (UniProt: P19388)
| PDB | External Database | Details |
|---|---|---|
| Phe 44 | Ser 44 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 654698.5 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 974.8 | |
| All* | Total formula weight | 655673.3 |
