9EH2
RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-FACT nucleosome upstream
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase subunit | polymer | 1984 | 219049.5 | 1 | UniProt (A0A7M4DUC2) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) | Sus scrofa (pig) | |
| 2 | B (B) | DNA-directed RNA polymerase subunit beta | polymer | 1251 | 142426.1 | 1 | UniProt (A0A4X1TVZ5) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (A0A0B8RVL1) | Sus scrofa (pig) | |
| 3 | C (C) | DNA-directed RNA polymerase II subunit RPB3 | polymer | 275 | 31439.1 | 1 | UniProt (A0A481DF93) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (I3LCH3) | Sus scrofa (pig) | |
| 4 | D (D) | RNA polymerase Rpb4/RPC9 core domain-containing protein | polymer | 142 | 16331.3 | 1 | UniProt (A0A8D0KES4) | Sus scrofa (pig) | |
| 5 | E (E) | DNA-directed RNA polymerase II subunit E | polymer | 210 | 24644.3 | 1 | UniProt (A0A4X1VTX4) Pfam (PF03871) Pfam (PF01191) UniProt (by SIFTS) (I3LSI7) | Sus scrofa (pig) | RPB5 |
| 6 | F (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 127 | 14477.0 | 1 | UniProt (A0A4X1VEK9) Pfam (PF01192) | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit F,RPB6 homolog |
| 7 | G (G) | DNA-directed RNA polymerase II subunit RPB7 | polymer | 172 | 19314.3 | 1 | UniProt (A0A4X1VKG7) Pfam (PF03876) Pfam (PF00575) | Sus scrofa (pig) | |
| 8 | H (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 150 | 17162.3 | 1 | UniProt (A0A8D1AQR7) Pfam (PF03870) | Sus scrofa (pig) | |
| 9 | I (I) | DNA-directed RNA polymerase II subunit RPB9 | polymer | 125 | 14541.2 | 1 | UniProt (P60899) Pfam (PF02150) Pfam (PF01096) | Sus scrofa (pig) | RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5 |
| 10 | J (J) | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 67 | 7655.1 | 1 | UniProt (A0A8W4F9W9) Pfam (PF01194) | Sus scrofa (pig) | |
| 11 | K (K) | RNA polymerase II subunit J | polymer | 117 | 13310.3 | 1 | UniProt (A0A8D0TE17) Pfam (PF13656) | Sus scrofa (pig) | |
| 12 | L (L) | RNA polymerase II subunit K | polymer | 58 | 7018.2 | 1 | UniProt (A0A4X1TRS6) Pfam (PF03604) | Sus scrofa (pig) | |
| 13 | M (M) | Transcription elongation factor SPT6 | polymer | 1729 | 199603.0 | 1 | UniProt (Q7KZ85) Pfam (PF14632) Pfam (PF14641) Pfam (PF22706) Pfam (PF14639) Pfam (PF14635) Pfam (PF17674) Pfam (PF00575) Pfam (PF14633) | Homo sapiens (human) | hSPT6,Histone chaperone suppressor of Ty6,Tat-cotransactivator 2 protein,Tat-CT2 protein |
| 14 | N (N) | non-template DNA | polymer | 205 | 63465.1 | 1 | synthetic construct | ||
| 15 | O (O) | Protein IWS1 homolog | polymer | 821 | 92235.9 | 1 | UniProt (Q96ST2) Pfam (PF08711) | Homo sapiens (human) | IWS1-like protein |
| 16 | P (P) | RNA | polymer | 11 | 3478.0 | 1 | synthetic construct | ||
| 17 | Q (Q) | RNA polymerase-associated protein CTR9 homolog | polymer | 1179 | 134510.2 | 1 | UniProt (Q6PD62) Pfam (PF13374) Pfam (PF13181) | Homo sapiens (human) | SH2 domain-binding protein 1 |
| 18 | R (R) | RNA polymerase-associated protein RTF1 homolog | polymer | 713 | 80733.0 | 1 | UniProt (Q92541) Pfam (PF03126) | Homo sapiens (human) | |
| 19 | S (S) | Transcription elongation factor A protein 1 | polymer | 304 | 34295.0 | 1 | UniProt (P23193) Pfam (PF08711) Pfam (PF07500) Pfam (PF01096) | Homo sapiens (human) | TFIIS |
| 20 | T (T) | Template DNA | polymer | 215 | 66259.5 | 1 | synthetic construct | ||
| 21 | U (U) | RNA polymerase-associated protein LEO1 | polymer | 666 | 75514.2 | 1 | UniProt (Q8WVC0) Pfam (PF04004) | Homo sapiens (human) | Replicative senescence down-regulated leo1-like protein |
| 22 | V (V) | RNA polymerase II-associated factor 1 homolog | polymer | 531 | 60052.7 | 1 | UniProt (Q8N7H5) Pfam (PF03985) | Homo sapiens (human) | hPAF1,Pancreatic differentiation protein 2 |
| 23 | W (W) | WDR61 | polymer | 305 | 33617.5 | 1 | UniProt (Q9GZS3) Pfam (PF00400) | Homo sapiens (human) | |
| 24 | X (X) | Parafibromin | polymer | 531 | 60673.5 | 1 | UniProt (Q6P1J9) Pfam (PF16050) Pfam (PF05179) | Homo sapiens (human) | Cell division cycle protein 73 homolog,Hyperparathyroidism 2 protein |
| 25 | Y (Y) | Transcription elongation factor SPT4 | polymer | 121 | 13508.5 | 1 | UniProt (P63272) Pfam (PF06093) | Homo sapiens (human) | hSPT4,DRB sensitivity-inducing factor 14 kDa subunit,DSIF p14,DRB sensitivity-inducing factor small subunit,DSIF small subunit |
| 26 | Z (Z) | Transcription elongation factor SPT5 | polymer | 1087 | 121225.5 | 1 | UniProt (O00267) Pfam (PF11942) Pfam (PF03439) Pfam (PF00467) | Homo sapiens (human) | hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein |
| 27 | AA, CA (a, e) | Histone H3 | polymer | 136 | 15437.1 | 2 | UniProt (A0A310TTQ1) Pfam (PF00125) | Xenopus laevis (African clawed frog) | |
| 28 | BA, DA (b, f) | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 29 | EA (g) | Histone H2A type 1 | polymer | 130 | 14109.4 | 1 | UniProt (P06897) Pfam (PF00125) Pfam (PF16211) | Xenopus laevis (African clawed frog) | |
| 30 | FA (h) | Histone H2B 1.1 | polymer | 123 | 13655.9 | 1 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 31 | GA (l) | Histone-lysine N-methyltransferase SETD2 | polymer | 1133 | 127887.3 | 1 | UniProt (Q9BYW2) Pfam (PF08236) Pfam (PF17907) Pfam (PF00856) Pfam (PF00397) | Homo sapiens (human) | HIF-1,Huntingtin yeast partner B,Huntingtin-interacting protein 1,HIP-1,Huntingtin-interacting protein B,Lysine N-methyltransferase 3A,Protein-lysine N-methyltransferase SETD2,SET domain-containing protein 2,hSET2,p231HBP |
| 32 | HA (x) | FACT complex subunit SPT16 | polymer | 1049 | 120291.0 | 1 | UniProt (Q9Y5B9) Pfam (PF14826) Pfam (PF00557) Pfam (PF08644) Pfam (PF08512) Pfam (PF21091) | Homo sapiens (human) | Chromatin-specific transcription elongation factor 140 kDa subunit,FACT 140 kDa subunit,FACTp140,Facilitates chromatin transcription complex subunit SPT16,hSPT16 |
| 33 | IA (y) | FACT complex subunit SSRP1 | polymer | 709 | 81207.1 | 1 | UniProt (Q08945) Pfam (PF17292) Pfam (PF03531) Pfam (PF21103) Pfam (PF08512) Pfam (PF00505) Pfam (PF21092) | Homo sapiens (human) | Chromatin-specific transcription elongation factor 80 kDa subunit,Facilitates chromatin transcription complex 80 kDa subunit,FACT 80 kDa subunit,FACTp80,Facilitates chromatin transcription complex subunit SSRP1,Recombination signal sequence recognition protein 1,Structure-specific recognition protein 1,hSSRP1,T160 |
| 34 | JA, KA, MA, NA, OA... (A, B, C, I, J...) | ZINC ION | non-polymer | 65.4 | 9 | Chemie (ZN) | |||
| 35 | LA (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
M: 1 - 1726 (UniProt: Q7KZ85)
O: 2 - 819 (UniProt: Q96ST2)
Q: 1 - 1173 (UniProt: Q6PD62)
R: 1 - 710 (UniProt: Q92541)
S: 1 - 301 (UniProt: P23193)
Y: 1 - 117 (UniProt: P63272)
a, e: 0 - 135 (UniProt: A0A310TTQ1)
g: 0 - 129 (UniProt: P06897)
h: 4 - 125 (UniProt: P02281)
l: 1435 - 2564 (UniProt: Q9BYW2)
x: 1 - 1047 (UniProt: Q9Y5B9)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser -1 | - | expression tag |
| Asn 0 | - | expression tag |
| Ala 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Glu 1174 | - | expression tag |
| Asn 1175 | - | expression tag |
| Leu 1176 | - | expression tag |
| Tyr 1177 | - | expression tag |
| Phe 1178 | - | expression tag |
| Gln 1179 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 36 | Lys 37 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Arg 99 | Gly 100 | conflict |
| Ser 123 | Ala 124 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met 3 | - | initiating methionine |
| Thr 32 | Ser 33 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 1432 | - | expression tag |
| Asn 1433 | - | expression tag |
| Ala 1434 | - | expression tag |
| Leu 1962 | Pro 1962 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser -1 | - | expression tag |
| Asn 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 35 |
| Total formula weight | 1977354.1 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 613.0 | |
| All* | Total formula weight | 1977967.1 |
