9EG8
Cryo-EM structure of COP9 signalosome precatalytic state with neddylated cullin-4A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (E) | COP9 signalosome complex subunit 5 | polymer | 334 | 37562.7 | 1 | UniProt (Q92905) Pfam (PF01398) Pfam (PF18323) | Homo sapiens (human) | SGN5,Signalosome subunit 5,Jun activation domain-binding protein 1 |
| 2 | B (I) | NEDD8 | polymer | 81 | 9086.6 | 1 | UniProt (Q15843) Pfam (PF00240) | Homo sapiens (human) | Neddylin,Neural precursor cell expressed developmentally down-regulated protein 8,NEDD-8,Ubiquitin-like protein Nedd8 |
| 3 | C (J) | Cullin-4A | polymer | 759 | 87814.3 | 1 | UniProt (Q13619) Pfam (PF00888) Pfam (PF10557) | Homo sapiens (human) | CUL-4A |
| 4 | D (A) | COP9 signalosome complex subunit 1 | polymer | 491 | 55606.5 | 1 | UniProt (Q13098) Pfam (PF10602) Pfam (PF01399) Pfam (PF21151) | Homo sapiens (human) | SGN1,Signalosome subunit 1,G protein pathway suppressor 1,GPS-1,JAB1-containing signalosome subunit 1,Protein MFH |
| 5 | E (B) | COP9 signalosome complex subunit 2 | polymer | 443 | 51664.6 | 1 | UniProt (P61201) Pfam (PF01399) | Homo sapiens (human) | SGN2,Signalosome subunit 2,Alien homolog,JAB1-containing signalosome subunit 2,Thyroid receptor-interacting protein 15,TR-interacting protein 15,TRIP-15 |
| 6 | F (C) | COP9 signalosome complex subunit 3 | polymer | 423 | 47924.0 | 1 | UniProt (Q9UNS2) Pfam (PF22788) Pfam (PF01399) Pfam (PF21215) | Homo sapiens (human) | SGN3,Signalosome subunit 3,JAB1-containing signalosome subunit 3 |
| 7 | G (D) | COP9 signalosome complex subunit 4 | polymer | 406 | 46322.7 | 1 | UniProt (Q9BT78) Pfam (PF18420) Pfam (PF22241) Pfam (PF01399) | Homo sapiens (human) | SGN4,Signalosome subunit 4,JAB1-containing signalosome subunit 4 |
| 8 | H (F) | COP9 signalosome complex subunit 6 | polymer | 327 | 36203.4 | 1 | UniProt (Q7L5N1) Pfam (PF01398) Pfam (PF13012) | Homo sapiens (human) | SGN6,Signalosome subunit 6,JAB1-containing signalosome subunit 6,MOV34 homolog,Vpr-interacting protein,hVIP |
| 9 | I (G) | COP9 signalosome complex subunit 7b | polymer | 264 | 29656.9 | 1 | UniProt (Q9H9Q2) Pfam (PF22061) Pfam (PF01399) Pfam (PF18392) | Homo sapiens (human) | SGN7b,Signalosome subunit 7b,JAB1-containing signalosome subunit 7b |
| 10 | J (H) | COP9 signalosome complex subunit 8 | polymer | 209 | 23245.5 | 1 | UniProt (Q99627) Pfam (PF10075) | Homo sapiens (human) | SGN8,Signalosome subunit 8,COP9 homolog,hCOP9,JAB1-containing signalosome subunit 8 |
| 11 | K (K) | E3 ubiquitin-protein ligase RBX1 | polymer | 108 | 12290.0 | 1 | UniProt (P62877) Pfam (PF12678) | Homo sapiens (human) | E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1 |
| 12 | L, M, N (K) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) |
Sequence modifications
E: 1 - 334 (UniProt: Q92905)
| PDB | External Database | Details |
|---|---|---|
| Ala 76 | Glu 76 | engineered mutation |
| Asn 151 | Asp 151 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 437377.2 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 196.2 | |
| All* | Total formula weight | 437573.4 |
