9EE9

Cryo-EM structure of the adenosine A2A receptor intermediate bound to a miniGs heterotrimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Adenosine receptor A2a	polymer	353	39517.1	1	UniProt (P29274) Pfam (PF00001)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37728.2	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (D)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	263	30776.7	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
5	E (E)	nanobody Nb35	polymer	156	16926.1	1		Lama glama (llama)
6	F (A)	ADENOSINE	non-polymer		267.2	1	Chemie (ADN)

Sequence modifications

A: 2 - 316 (UniProt: P29274)

PDB	External Database	Details
Asp -26	-	expression tag
Tyr -25	-	expression tag
Lys -24	-	expression tag
Asp -23	-	expression tag
Asp -22	-	expression tag
Asp -21	-	expression tag
Asp -20	-	expression tag
Lys -19	-	expression tag
Ser -18	-	expression tag
Asn -17	-	expression tag
Asn -16	-	expression tag
Asn -15	-	expression tag
Asn -14	-	expression tag
Asn -13	-	expression tag
Asn -12	-	expression tag
Asn -11	-	expression tag
Asn -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Gly -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ala 1	-	expression tag
Cys 229	Val 229	engineered mutation
Ala 291	Arg 291	engineered mutation
His 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

D: 5 - 195 (UniProt: P63092)

PDB	External Database	Details
Met -9	-	initiating methionine
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Glu -1	-	expression tag
Asn 0	-	expression tag
Leu 1	-	expression tag
Tyr 2	-	expression tag
Phe 3	-	expression tag
Gln 4	-	expression tag
Asp 49	Gly 49	conflict
Asn 50	Glu 50	conflict

D: 204 - 384 (UniProt: P63092)

PDB	External Database	Details
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 249	conflict
Asp 252	Ser 252	conflict
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Ala 362	Ile 372	conflict
Ile 365	Val 375	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	132809.2
Non-Polymers*	Number of molecules	1
	Total formula weight	267.2
All*	Total formula weight	133076.4

*Water molecules are not included.