9EDT

Tubulin cofactors D,E,G bound to tubulin dimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (D)	Chromosome instability protein 1	polymer	1020	117608.2	1	UniProt (P40987)	Saccharomyces cerevisiae (brewer's yeast)
2	B (G)	GTP-binding protein CIN4	polymer	191	22096.7	1	UniProt (P39110)	Saccharomyces cerevisiae (brewer's yeast)	Chromosome instability protein 4
3	C (A)	Tubulin alpha-1A chain	polymer	451	50121.3	1	UniProt (P02550)	Sus scrofa (pig)	Alpha-tubulin 1,Tubulin alpha-1 chain
4	D (B)	Tubulin beta chain	polymer	445	49907.8	1	UniProt (P02554)	Sus scrofa (pig)	Beta-tubulin
5	E (F)	Designed ankyrin repeat protein -- Darpin	polymer	169	17955.3	1		Escherichia coli
6	F (E)	Protein PAC2	polymer	459	41916.7	1	UniProt (P39937)	Saccharomyces cerevisiae (brewer's yeast)
7	G, H (G, A)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
8	I (B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)

Sequence modifications

D: 2 - 1014 (UniProt: P40987)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

E: 1 - 406 (PDB: 9EDT)
E: 422 - 518 (UniProt: P39937)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	299605.9
Non-Polymers*	Number of molecules	3
	Total formula weight	1489.6
All*	Total formula weight	301095.5

*Water molecules are not included.