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9EDA

SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State IV - product)

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(B)
sgRNApolymer9731335.61synthetic construct
2B
(C)
PAM-proximal Target Strandpolymer226743.41synthetic construct
3C
(D)
Non-target strandpolymer5517027.01synthetic construct
4D
(c)
PAM-distal Target Strandpolymer3310077.51synthetic construct
5E
(A)
CRISPR-associated endonuclease Cas9/Csn1polymer1368158699.81UniProt (Q99ZW2)
Pfam (PF16593)
Pfam (PF16592)
Pfam (PF13395)
Pfam (PF22702)
Pfam (PF16595)
Streptococcus pyogenesSpCas9,SpyCas9
6F
(A)
waterwater18.01Chemie (HOH)
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains5
Total formula weight223883.3
All*Total formula weight223883.3
*Water molecules are not included.

247536

PDB entries from 2026-01-14

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