9EC5

Streptavidin-S112C-L124F bound to Cu(II)dpea cofactor, containing S-nitrosocysteine modification

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Streptavidin	polymer	159	16648.1	1	UniProt (P22629)	Streptomyces avidinii
2	B (A)	N-(3-{bis[2-(pyridin-2-yl)ethyl]amino}propyl)-5-[(3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide	non-polymer		510.7	1	Chemie (A1BIA)
3	C, D (A)	COPPER (II) ION	non-polymer		63.5	2	Chemie (CU)
4	E (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	F (A)	water	water		18.0	139	Chemie (HOH)

Sequence modifications

A: 14 - 159 (UniProt: P22629)

PDB	External Database	Details
Met 1	-	expression tag
Ala 2	-	expression tag
Ser 3	-	expression tag
Met 4	-	expression tag
Thr 5	-	expression tag
Gly 6	-	expression tag
Gly 7	-	expression tag
Gln 8	-	expression tag
Gln 9	-	expression tag
Met 10	-	expression tag
Gly 11	-	expression tag
Arg 12	-	expression tag
Asp 13	-	expression tag
Gln 14	Glu 38	engineered mutation
Snc 112	Ser 136	engineered mutation
Phe 124	Leu 148	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	16648.1
Non-Polymers*	Number of molecules	4
	Total formula weight	733.9
All*	Total formula weight	17382.0

*Water molecules are not included.