9EBH

Human adenosine A3 receptor Gi1 complex bound to adenosine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	349	38402.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (E)	Antibody fragment scFv16|Mus musculus (10090)	polymer	248	26466.5	1		Mus musculus
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (R)	Adenosine receptor A3	polymer	363	41203.6	1	UniProt (P0DMS8) Pfam (PF00001)	Homo sapiens (human)
6	F (R)	ADENOSINE	non-polymer		267.2	1	Chemie (ADN)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 2 - 318 (UniProt: P0DMS8)

PDB	External Database	Details
Asp -36	-	expression tag
Tyr -35	-	expression tag
Lys -34	-	expression tag
Asp -33	-	expression tag
Asp -32	-	expression tag
Asp -31	-	expression tag
Asp -30	-	expression tag
Ala -29	-	expression tag
Ala -28	-	expression tag
Asn -27	-	expression tag
Phe -26	-	expression tag
Thr -25	-	expression tag
Pro -24	-	expression tag
Val -23	-	expression tag
Asn -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
Gly -18	-	expression tag
Asn -17	-	expression tag
Gln -16	-	expression tag
Ser -15	-	expression tag
Val -14	-	expression tag
Arg -13	-	expression tag
Leu -12	-	expression tag
Val -11	-	expression tag
Thr -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Pro 1	-	expression tag
His 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	154217.0
Non-Polymers*	Number of molecules	1
	Total formula weight	267.2
All*	Total formula weight	154484.2

*Water molecules are not included.