9EAL
SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State II - precleavage)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A (B) | sgRNA | polymer | 97 | 31335.6 | 1 | synthetic construct | ||
2 | B (C) | DNA (Target Strand) | polymer | 55 | 16865.8 | 1 | synthetic construct | ||
3 | C (D) | DNA (Non-target strand) | polymer | 55 | 17027.0 | 1 | synthetic construct | ||
4 | D (A) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158699.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | SpCas9,SpyCas9 |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 223928.2 | |
All* | Total formula weight | 223928.2 |