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9EAL

SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State II - precleavage)

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(B)
sgRNApolymer9731335.61synthetic construct
2B
(C)
DNA (Target Strand)polymer5516865.81synthetic construct
3C
(D)
DNA (Non-target strand)polymer5517027.01synthetic construct
4D
(A)
CRISPR-associated endonuclease Cas9/Csn1polymer1368158699.81UniProt (Q99ZW2)
Pfam (PF16593)
Pfam (PF16592)
Pfam (PF13395)
Pfam (PF22702)
Pfam (PF16595)
Streptococcus pyogenesSpCas9,SpyCas9
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains4
Total formula weight223928.2
All*Total formula weight223928.2
*Water molecules are not included.

239149

PDB entries from 2025-07-23

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