9EAH
Structure of nanobody AT209 in complex with the olmesartan-bound angiotensin II type I receptor (AT1R)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, B) | Nanobody AT209,Type-1 angiotensin II receptor,Soluble cytochrome b562 | polymer | 580 | 65181.4 | 2 | UniProt (P30556) UniProt (P0ABE7) Pfam (PF00001) | Camelidae | AT1AR,AT1BR,Angiotensin II type-1 receptor,AT1 receptor,Cytochrome b-562 |
| 2 | B (C) | BAG2 Anti-BRIL Fab Heavy Chain | polymer | 231 | 24539.3 | 1 | synthetic construct | ||
| 3 | C (D) | BAG2 Anti-BRIL Fab Light Chain | polymer | 215 | 23541.2 | 1 | synthetic construct | ||
| 4 | E (A) | Olmesartan | non-polymer | 446.5 | 1 | Chemie (OLM) | |||
| 5 | F (A) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) |
Sequence modifications
A, B: -132 - -15 (PDB: 9EAH)
A, B: 2 - 226 (UniProt: P30556)
A, B: 227 - 331 (UniProt: P0ABE7)
A, B: 1235 - 1319 (UniProt: P30556)
| PDB | External Database | Details |
|---|---|---|
| Gly -14 | - | linker |
| Gly -13 | - | linker |
| Ser -12 | - | linker |
| Gly -11 | - | linker |
| Glu -10 | - | linker |
| Asp -9 | - | linker |
| Gln -8 | - | linker |
| Val -7 | - | linker |
| Asp -6 | - | linker |
| Pro -5 | - | linker |
| Arg -4 | - | linker |
| Leu -3 | - | linker |
| Ile -2 | - | linker |
| Asp -1 | - | linker |
| Gly 0 | - | linker |
| Lys 1 | - | linker |
A, B: 227 - 331 (UniProt: P0ABE7)
| PDB | External Database | Details |
|---|---|---|
| Trp 232 | Met 29 | engineered mutation |
| Ile 327 | His 124 | engineered mutation |
| Leu 331 | Arg 128 | engineered mutation |
| Glu 332 | - | linker |
| Arg 333 | - | linker |
| Ala 334 | - | linker |
| Arg 335 | - | linker |
| Ser 336 | - | linker |
| Thr 337 | - | linker |
| Leu 338 | - | linker |
| Asp 339 | - | linker |
| Lys 340 | - | linker |
| Leu 341 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 1320 | - | expression tag |
| Gly 1321 | - | expression tag |
| Ser 1322 | - | expression tag |
| Ser 1323 | - | expression tag |
| Leu 1324 | - | expression tag |
| Glu 1325 | - | expression tag |
| Val 1326 | - | expression tag |
| Leu 1327 | - | expression tag |
| Phe 1328 | - | expression tag |
| Gln 1329 | - | expression tag |
| Gly 1330 | - | expression tag |
| Pro 1331 | - | expression tag |
| Thr 1332 | - | expression tag |
| Glu 1333 | - | expression tag |
| Thr 1334 | - | expression tag |
| Ser 1335 | - | expression tag |
| Gln 1336 | - | expression tag |
| Val 1337 | - | expression tag |
| Ala 1338 | - | expression tag |
| Pro 1339 | - | expression tag |
| Ala 1340 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 178443.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 833.2 | |
| All* | Total formula weight | 179276.5 |
