9EAH
Structure of nanobody AT209 in complex with the olmesartan-bound angiotensin II type I receptor (AT1R)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, B) | Nanobody AT209,Type-1 angiotensin II receptor,Soluble cytochrome b562 | polymer | 580 | 65181.4 | 2 | UniProt (P30556) UniProt (P0ABE7) Pfam (PF00001) | Camelidae | AT1AR,AT1BR,Angiotensin II type-1 receptor,AT1 receptor,Cytochrome b-562 |
| 2 | B (C) | BAG2 Anti-BRIL Fab Heavy Chain | polymer | 231 | 24539.3 | 1 | synthetic construct | ||
| 3 | C (D) | BAG2 Anti-BRIL Fab Light Chain | polymer | 215 | 23541.2 | 1 | synthetic construct | ||
| 4 | E (A) | Olmesartan | non-polymer | 446.5 | 1 | Chemie (OLM) | |||
| 5 | F (A) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) PubChem (16219173) PubChem (16758052) PubChem (304) PubChem (176980071) PubChem (191463) PubChem (368063) PubChem (369319) PubChem (18640088) PubChem (1715243) PubChem (40477506) PubChem (40557614) PubChem (42579431) PubChem (42579432) PubChem (1715244) PubChem (23423981) PubChem (45044259) PubChem (638019) PubChem (49853509) PubChem (50990013) PubChem (44654663) PubChem (52934537) PubChem (56621683) PubChem (1549081) PubChem (1553803) PubChem (46937002) PubChem (57915018) PubChem (57939063) PubChem (58428240) PubChem (58445498) PubChem (58663286) PubChem (59450329) PubChem (59628412) PubChem (59965215) PubChem (71234641) PubChem (59993755) PubChem (60001463) PubChem (66634651) PubChem (59274407) PubChem (5283629) PubChem (5284644) PubChem (68055877) PubChem (68166463) PubChem (68315613) PubChem (5315842) PubChem (70795838) PubChem (70797612) PubChem (70954610) PubChem (70957185) PubChem (71463909) PubChem (68311425) PubChem (68311398) PubChem (143173313) PubChem (177156123) PubChem (6419195) PubChem (5997) PubChem (6994560) PubChem (7055705) PubChem (7055706) PubChem (7059398) PubChem (7059400) PubChem (7059635) PubChem (7103033) PubChem (7182477) PubChem (7182478) PubChem (89951918) PubChem (90224410) PubChem (91298236) PubChem (9909206) PubChem (10045721) PubChem (71168115) PubChem (10134774) PubChem (10293553) PubChem (71173031) PubChem (11003580) PubChem (11025495) PubChem (11058155) PubChem (11463329) PubChem (1551269) PubChem (90070115) PubChem (11868624) PubChem (11868625) PubChem (11869366) PubChem (11869367) PubChem (11869368) PubChem (11869369) PubChem (12120344) PubChem (12156156) PubChem (12303135) PubChem (12303148) PubChem (12303156) PubChem (12303159) PubChem (12303160) PubChem (117965027) PubChem (6432564) PubChem (101624109) PubChem (6710666) PubChem (124146240) PubChem (7059399) PubChem (124897607) PubChem (124896959) PubChem (10861996) PubChem (132837164) PubChem (11834483) PubChem (11888915) PubChem (11888914) PubChem (124225449) PubChem (124429284) PubChem (124909223) PubChem (124909224) PubChem (124909225) PubChem (138967089) PubChem (139566851) PubChem (130459183) PubChem (131992107) PubChem (15939944) PubChem (139568882) PubChem (142983773) PubChem (144054483) PubChem (144209461) PubChem (144331236) PubChem (144808354) PubChem (145053568) PubChem (145157652) PubChem (145215073) PubChem (145230478) PubChem (146320991) PubChem (147857230) PubChem (154993519) PubChem (171828367) PubChem (173516975) PubChem (158603858) PubChem (162906452) PubChem (162906453) PubChem (162906454) PubChem (162906455) PubChem (162906456) PubChem (162906457) PubChem (163255768) PubChem (163389210) PubChem (164665303) PubChem (166553771) PubChem (166712004) PubChem (170116175) PubChem (171764280) PubChem (171817101) PubChem (171817104) PubChem (174285573) |
Sequence modifications
A, B: -132 - -15 (PDB: 9EAH)
A, B: 2 - 226 (UniProt: P30556)
A, B: 227 - 331 (UniProt: P0ABE7)
A, B: 1235 - 1319 (UniProt: P30556)
| PDB | External Database | Details |
|---|---|---|
| Gly -14 | - | linker |
| Gly -13 | - | linker |
| Ser -12 | - | linker |
| Gly -11 | - | linker |
| Glu -10 | - | linker |
| Asp -9 | - | linker |
| Gln -8 | - | linker |
| Val -7 | - | linker |
| Asp -6 | - | linker |
| Pro -5 | - | linker |
| Arg -4 | - | linker |
| Leu -3 | - | linker |
| Ile -2 | - | linker |
| Asp -1 | - | linker |
| Gly 0 | - | linker |
| Lys 1 | - | linker |
A, B: 227 - 331 (UniProt: P0ABE7)
| PDB | External Database | Details |
|---|---|---|
| Trp 232 | Met 29 | engineered mutation |
| Ile 327 | His 124 | engineered mutation |
| Leu 331 | Arg 128 | engineered mutation |
| Glu 332 | - | linker |
| Arg 333 | - | linker |
| Ala 334 | - | linker |
| Arg 335 | - | linker |
| Ser 336 | - | linker |
| Thr 337 | - | linker |
| Leu 338 | - | linker |
| Asp 339 | - | linker |
| Lys 340 | - | linker |
| Leu 341 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 1320 | - | expression tag |
| Gly 1321 | - | expression tag |
| Ser 1322 | - | expression tag |
| Ser 1323 | - | expression tag |
| Leu 1324 | - | expression tag |
| Glu 1325 | - | expression tag |
| Val 1326 | - | expression tag |
| Leu 1327 | - | expression tag |
| Phe 1328 | - | expression tag |
| Gln 1329 | - | expression tag |
| Gly 1330 | - | expression tag |
| Pro 1331 | - | expression tag |
| Thr 1332 | - | expression tag |
| Glu 1333 | - | expression tag |
| Thr 1334 | - | expression tag |
| Ser 1335 | - | expression tag |
| Gln 1336 | - | expression tag |
| Val 1337 | - | expression tag |
| Ala 1338 | - | expression tag |
| Pro 1339 | - | expression tag |
| Ala 1340 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 178443.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 833.2 | |
| All* | Total formula weight | 179276.5 |
