9EA3
T4 bacteriophage replicative holoenzyme containing triple mutations D75R, Q430E, and K432E in the exonuclease-deficient polymerase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed DNA polymerase | polymer | 898 | 103745.8 | 1 | UniProt (A0A7S9SVB6) | Escherichia phage T4 | |
| 2 | B, C, D (B, D, E) | Sliding clamp | polymer | 228 | 24881.2 | 3 | UniProt (P04525) | Escherichia phage T4 | DNA polymerase accessory protein Gp45,DNA polymerase clamp,Gene product 45,gp45,Sliding clamp Gp45 |
| 3 | E (P) | DNA (5'-AGC TAT GAC CAT GAT TAC GAA TTG ddC-3') | polymer | 25 | 7682.0 | 1 | synthetic construct | ||
| 4 | F (T) | DNA (38-MER) | polymer | 38 | 11677.5 | 1 | synthetic construct |
Sequence modifications
A: 1 - 898 (UniProt: A0A7S9SVB6)
| PDB | External Database | Details |
|---|---|---|
| Arg 75 | Asp 75 | engineered mutation |
| Ala 219 | Asp 219 | conflict |
| Glu 430 | Gln 430 | engineered mutation |
| Glu 432 | Lys 432 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 197749.0 | |
| All* | Total formula weight | 197749.0 |
