9E97

L-allo-threonine aldolase from Thermotoga maritima N308E-Y87A-R122G-P121D Mutant with a 2-(aminomethyl)pyridine PLP modification

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	L-allo-threonine aldolase	polymer	349	38218.6	2	UniProt (Q9X266) Pfam (PF01212)	Thermotoga maritima
2	C, J (A, B)	{5-hydroxy-6-methyl-4-[(E)-{[(pyridin-2-yl)methyl]imino}methyl]pyridin-3-yl}methyl dihydrogen phosphate	non-polymer		337.3	2	Chemie (A1BGC)
3	D, K (A, B)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
4	E (A)	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
5	F, G, L (A, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
6	H, I, M, N (A, B)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
7	O, P (A, B)	water	water		18.0	341	Chemie (HOH)

Sequence modifications

A, B: 1 - 341 (UniProt: Q9X266)

PDB	External Database	Details
Ala 87	Tyr 87	engineered mutation
Asp 121	Pro 121	engineered mutation
Gly 122	Arg 122	engineered mutation
Glu 308	Asn 308	engineered mutation
Ser 340	Arg 340	engineered mutation
Gln 341	Lys 341	engineered mutation
Val 342	-	expression tag
Gln 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76437.1
Non-Polymers*	Number of molecules	12
	Total formula weight	1779.9
All*	Total formula weight	78217.1

*Water molecules are not included.