9E8O
Nub1/Fat10-processing human 26S proteasome bound to Txnl1 with Rpt2 at top of spiral staircase and partially unfolded Eos
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | 26S proteasome regulatory subunit 4 | polymer | 440 | 49260.5 | 1 | UniProt (P62191) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (human) | P26s4,26S proteasome AAA-ATPase subunit RPT2,Proteasome 26S subunit ATPase 1 |
| 2 | B (C) | 26S protease regulatory subunit 8 | polymer | 406 | 45694.0 | 1 | UniProt (P62195) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (human) | 26S proteasome AAA-ATPase subunit RPT6,Proteasome 26S subunit ATPase 5,Proteasome subunit p45,Thyroid hormone receptor-interacting protein 1,TRIP1,p45/SUG |
| 3 | C (D) | 26S proteasome regulatory subunit 6B | polymer | 418 | 47426.1 | 1 | UniProt (P43686) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (human) | 26S proteasome AAA-ATPase subunit RPT3,MB67-interacting protein,MIP224,Proteasome 26S subunit ATPase 4,Tat-binding protein 7,TBP-7 |
| 4 | D (c) | 26S proteasome non-ATPase regulatory subunit 14 | polymer | 424 | 46940.9 | 1 | UniProt (O00487) Pfam (PF01398) | Homo sapiens (human) | |
| 5 | E (G) | Proteasome subunit alpha type-6 | polymer | 246 | 27432.5 | 1 | UniProt (P60900) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (human) | 27 kDa prosomal protein,p27K,Macropain iota chain,Multicatalytic endopeptidase complex iota chain,Proteasome iota chain |
| 6 | F (H) | Proteasome subunit alpha type-2 | polymer | 234 | 25927.5 | 1 | UniProt (P25787) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (human) | Macropain subunit C3,Multicatalytic endopeptidase complex subunit C3,Proteasome component C3 |
| 7 | G (I) | Proteasome subunit alpha type-4 | polymer | 261 | 29525.8 | 1 | UniProt (P25789) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (human) | Macropain subunit C9,Multicatalytic endopeptidase complex subunit C9,Proteasome component C9,Proteasome subunit L |
| 8 | H (J) | Proteasome subunit alpha type-7 | polymer | 248 | 27929.9 | 1 | UniProt (O14818) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (human) | Proteasome subunit RC6-1,Proteasome subunit XAPC7,Proteasome subunit alpha-4,alpha-4 |
| 9 | I (K) | Proteasome subunit alpha type-5 | polymer | 241 | 26436.0 | 1 | UniProt (P28066) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (human) | Macropain zeta chain,Multicatalytic endopeptidase complex zeta chain,Proteasome subunit alpha-5,alpha-5,Proteasome zeta chain |
| 10 | J (L) | Proteasome subunit alpha type-1 | polymer | 263 | 29595.6 | 1 | UniProt (P25786) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (human) | 30 kDa prosomal protein,PROS-30,Macropain subunit C2,Multicatalytic endopeptidase complex subunit C2,Proteasome component C2,Proteasome nu chain |
| 11 | K (M) | Proteasome subunit alpha type-3 | polymer | 255 | 28469.3 | 1 | UniProt (P25788) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (human) | Macropain subunit C8,Multicatalytic endopeptidase complex subunit C8,Proteasome component C8 |
| 12 | L (N) | Proteasome subunit beta type-6 | polymer | 239 | 25377.7 | 1 | UniProt (P28072) Pfam (PF00227) | Homo sapiens (human) | Macropain delta chain,Multicatalytic endopeptidase complex delta chain,Proteasome delta chain,Proteasome subunit Y |
| 13 | M (O) | Proteasome subunit beta type-7 | polymer | 277 | 30000.4 | 1 | UniProt (Q99436) Pfam (PF00227) Pfam (PF12465) | Homo sapiens (human) | Macropain chain Z,Multicatalytic endopeptidase complex chain Z,Proteasome subunit Z |
| 14 | N (P) | Proteasome subunit beta type-3 | polymer | 205 | 22972.9 | 1 | UniProt (P49720) Pfam (PF00227) | Homo sapiens (human) | Proteasome chain 13,Proteasome component C10-II,Proteasome theta chain |
| 15 | O (Q) | Proteasome subunit beta type-2 | polymer | 201 | 22864.3 | 1 | UniProt (P49721) Pfam (PF00227) | Homo sapiens (human) | Macropain subunit C7-I,Multicatalytic endopeptidase complex subunit C7-I,Proteasome component C7-I |
| 16 | P (R) | Proteasome subunit beta type-5 | polymer | 263 | 28510.2 | 1 | UniProt (P28074) Pfam (PF00227) | Homo sapiens (human) | Macropain epsilon chain,Multicatalytic endopeptidase complex epsilon chain,Proteasome chain 6,Proteasome epsilon chain,Proteasome subunit MB1,Proteasome subunit X |
| 17 | Q (S) | Proteasome subunit beta type-1 | polymer | 241 | 26522.4 | 1 | UniProt (P20618) Pfam (PF00227) | Homo sapiens (human) | Macropain subunit C5,Multicatalytic endopeptidase complex subunit C5,Proteasome component C5,Proteasome gamma chain |
| 18 | R (T) | Proteasome subunit beta type-4 | polymer | 264 | 29231.2 | 1 | UniProt (P28070) Pfam (PF00227) | Homo sapiens (human) | 26 kDa prosomal protein,PROS-26,Macropain beta chain,Multicatalytic endopeptidase complex beta chain,Proteasome beta chain,Proteasome chain 3,HsN3 |
| 19 | S (X) | 26S proteasome non-ATPase regulatory subunit 11 | polymer | 422 | 47526.7 | 1 | UniProt (O00231) Pfam (PF18055) Pfam (PF01399) Pfam (PF18503) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN6,26S proteasome regulatory subunit S9,26S proteasome regulatory subunit p44.5 |
| 20 | T (Y) | 26S proteasome non-ATPase regulatory subunit 6 | polymer | 389 | 45592.3 | 1 | UniProt (Q15008) Pfam (PF10602) Pfam (PF01399) Pfam (PF21154) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN7,26S proteasome regulatory subunit S10,Breast cancer-associated protein SGA-113M,Phosphonoformate immuno-associated protein 4,Proteasome regulatory particle subunit p44S10,p42A |
| 21 | U (Z) | 26S proteasome non-ATPase regulatory subunit 7 | polymer | 324 | 37086.4 | 1 | UniProt (P51665) Pfam (PF01398) Pfam (PF13012) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN8,26S proteasome regulatory subunit S12,Mov34 protein homolog,Proteasome subunit p40 |
| 22 | V (a) | 26S proteasome non-ATPase regulatory subunit 13 | polymer | 376 | 42995.4 | 1 | UniProt (Q9UNM6) Pfam (PF22037) Pfam (PF01399) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN9,26S proteasome regulatory subunit S11,26S proteasome regulatory subunit p40.5 |
| 23 | W (b) | 26S proteasome non-ATPase regulatory subunit 4 | polymer | 377 | 40781.6 | 1 | UniProt (P55036) Pfam (PF13519) Pfam (PF02809) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN10,26S proteasome regulatory subunit S5A,Antisecretory factor 1,ASF,Multiubiquitin chain-binding protein |
| 24 | X (d) | 26S proteasome non-ATPase regulatory subunit 8 | polymer | 350 | 39667.9 | 1 | UniProt (P48556) Pfam (PF10075) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN12,26S proteasome regulatory subunit S14,p31 |
| 25 | Y (f) | 26S proteasome non-ATPase regulatory subunit 2 | polymer | 908 | 100313.6 | 1 | UniProt (Q13200) Pfam (PF17781) Pfam (PF01851) Pfam (PF18051) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN1,26S proteasome regulatory subunit S2,26S proteasome subunit p97,Protein 55.11,Tumor necrosis factor type 1 receptor-associated protein 2 |
| 26 | Z (W) | 26S proteasome non-ATPase regulatory subunit 12 | polymer | 456 | 52979.4 | 1 | UniProt (O00232) Pfam (PF22241) Pfam (PF01399) Pfam (PF18098) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN5,26S proteasome regulatory subunit p55 |
| 27 | AA (V) | 26S proteasome non-ATPase regulatory subunit 3 | polymer | 534 | 61066.5 | 1 | UniProt (O43242) Pfam (PF01399) Pfam (PF08375) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN3,26S proteasome regulatory subunit S3,Proteasome subunit p58 |
| 28 | BA (e) | 26S proteasome complex subunit SEM1 | polymer | 70 | 8284.6 | 1 | UniProt (P60896) Pfam (PF05160) | Homo sapiens (human) | 26S proteasome complex subunit DSS1,Deleted in split hand/split foot protein 1,Split hand/foot deleted protein 1,Split hand/foot malformation type 1 protein |
| 29 | CA (A) | 26S proteasome regulatory subunit 7 | polymer | 433 | 48700.8 | 1 | UniProt (P35998) Pfam (PF21236) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (human) | 26S proteasome AAA-ATPase subunit RPT1,Proteasome 26S subunit ATPase 2,Protein MSS1 |
| 30 | DA (F) | 26S proteasome regulatory subunit 6A | polymer | 439 | 49266.5 | 1 | UniProt (P17980) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (human) | 26S proteasome AAA-ATPase subunit RPT5,Proteasome 26S subunit ATPase 3,Proteasome subunit P50,Tat-binding protein 1,TBP-1 |
| 31 | EA (E) | 26S protease regulatory subunit 10B | polymer | 389 | 44241.0 | 1 | UniProt (P62333) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (human) | 26S proteasome AAA-ATPase subunit RPT4,Proteasome 26S subunit ATPase 6,Proteasome subunit p42 |
| 32 | FA (U) | 26S proteasome non-ATPase regulatory subunit 1 | polymer | 953 | 105957.3 | 1 | UniProt (Q99460) Pfam (PF21505) Pfam (PF01851) Pfam (PF13646) Pfam (PF18004) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN2,26S proteasome regulatory subunit S1,26S proteasome subunit p112 |
| 33 | GA (g) | FAT10 | polymer | 392 | 44409.9 | 1 | UniProt (O15205) UniProt (Q5S6Z9) Pfam (PF00240) Pfam (PF01353) | Homo sapiens (human) | |
| 34 | HA (u) | Thioredoxin-like protein 1 | polymer | 300 | 33607.6 | 1 | UniProt (O43396) Pfam (PF00085) Pfam (PF06201) | Homo sapiens (human) | 32 kDa thioredoxin-related protein |
| 35 | IA, KA (B, C) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 36 | JA, LA, NA (B, C, D) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 37 | MA, PA, QA, RA (D, A, F, E) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 4 | Chemie (ADP) | |||
| 38 | OA (c) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
c: 1 - 310 (UniProt: O00487)
U: 1 - 953 (UniProt: Q99460)
g: 1 - 165 (UniProt: O15205)
g: 166 - 391 (UniProt: Q5S6Z9)
u: 1 - 289 (UniProt: O43396)
| PDB | External Database | Details |
|---|---|---|
| Leu 311 | - | expression tag |
| Ile 312 | - | expression tag |
| Asn 313 | - | expression tag |
| His 314 | - | expression tag |
| His 315 | - | expression tag |
| His 316 | - | expression tag |
| His 317 | - | expression tag |
| His 318 | - | expression tag |
| His 319 | - | expression tag |
| Asp 320 | - | expression tag |
| Tyr 321 | - | expression tag |
| Asp 322 | - | expression tag |
| Ile 323 | - | expression tag |
| Pro 324 | - | expression tag |
| Thr 325 | - | expression tag |
| Thr 326 | - | expression tag |
| Ala 327 | - | expression tag |
| Ser 328 | - | expression tag |
| Glu 329 | - | expression tag |
| Asn 330 | - | expression tag |
| Leu 331 | - | expression tag |
| Tyr 332 | - | expression tag |
| Phe 333 | - | expression tag |
| Gln 334 | - | expression tag |
| Gly 335 | - | expression tag |
| Glu 336 | - | expression tag |
| Leu 337 | - | expression tag |
| Gly 338 | - | expression tag |
| Met 339 | - | expression tag |
| Arg 340 | - | expression tag |
| Gly 341 | - | expression tag |
| Ser 342 | - | expression tag |
| Ala 343 | - | expression tag |
| Gly 344 | - | expression tag |
| Lys 345 | - | expression tag |
| Ala 346 | - | expression tag |
| Gly 347 | - | expression tag |
| Glu 348 | - | expression tag |
| Gly 349 | - | expression tag |
| Glu 350 | - | expression tag |
| Ile 351 | - | expression tag |
| Pro 352 | - | expression tag |
| Ala 353 | - | expression tag |
| Pro 354 | - | expression tag |
| Leu 355 | - | expression tag |
| Ala 356 | - | expression tag |
| Gly 357 | - | expression tag |
| Thr 358 | - | expression tag |
| Val 359 | - | expression tag |
| Ser 360 | - | expression tag |
| Lys 361 | - | expression tag |
| Ile 362 | - | expression tag |
| Leu 363 | - | expression tag |
| Val 364 | - | expression tag |
| Lys 365 | - | expression tag |
| Glu 366 | - | expression tag |
| Gly 367 | - | expression tag |
| Asp 368 | - | expression tag |
| Thr 369 | - | expression tag |
| Val 370 | - | expression tag |
| Lys 371 | - | expression tag |
| Ala 372 | - | expression tag |
| Gly 373 | - | expression tag |
| Gln 374 | - | expression tag |
| Thr 375 | - | expression tag |
| Val 376 | - | expression tag |
| Leu 377 | - | expression tag |
| Val 378 | - | expression tag |
| Leu 379 | - | expression tag |
| Glu 380 | - | expression tag |
| Ala 381 | - | expression tag |
| Met 382 | - | expression tag |
| Lys 383 | - | expression tag |
| Met 384 | - | expression tag |
| Glu 385 | - | expression tag |
| Thr 386 | - | expression tag |
| Glu 387 | - | expression tag |
| Ile 388 | - | expression tag |
| Asn 389 | - | expression tag |
| Ala 390 | - | expression tag |
| Pro 391 | - | expression tag |
| Thr 392 | - | expression tag |
| Asp 393 | - | expression tag |
| Gly 394 | - | expression tag |
| Lys 395 | - | expression tag |
| Val 396 | - | expression tag |
| Glu 397 | - | expression tag |
| Lys 398 | - | expression tag |
| Val 399 | - | expression tag |
| Leu 400 | - | expression tag |
| Val 401 | - | expression tag |
| Lys 402 | - | expression tag |
| Glu 403 | - | expression tag |
| Arg 404 | - | expression tag |
| Asp 405 | - | expression tag |
| Ala 406 | - | expression tag |
| Val 407 | - | expression tag |
| Gln 408 | - | expression tag |
| Gly 409 | - | expression tag |
| Gly 410 | - | expression tag |
| Gln 411 | - | expression tag |
| Gly 412 | - | expression tag |
| Leu 413 | - | expression tag |
| Ile 414 | - | expression tag |
| Lys 415 | - | expression tag |
| Ile 416 | - | expression tag |
| Gly 417 | - | expression tag |
| Val 418 | - | expression tag |
| His 419 | - | expression tag |
| His 420 | - | expression tag |
| His 421 | - | expression tag |
| His 422 | - | expression tag |
| His 423 | - | expression tag |
| His 424 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 320 | Asp 320 | conflict |
g: 166 - 391 (UniProt: Q5S6Z9)
| PDB | External Database | Details |
|---|---|---|
| Lys 176 | Asn 11 | conflict |
| Cr8 228 | His 62 | conflict |
| - | Tyr 63 | deletion |
| - | Gly 64 | deletion |
| Lys 235 | Glu 70 | conflict |
| Asn 239 | His 74 | conflict |
| Asn 267 | Ile 102 | conflict |
| Tyr 286 | His 121 | conflict |
| Thr 288 | Val 123 | conflict |
| Glu 323 | Thr 158 | conflict |
| Ala 354 | Tyr 189 | conflict |
| Tyr 392 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -10 | - | expression tag |
| His -9 | - | expression tag |
| Met -8 | - | expression tag |
| Asp -7 | - | expression tag |
| Tyr -6 | - | expression tag |
| Lys -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Lys 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 34 |
| Total formula weight | 1372594.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 2861.5 | |
| All* | Total formula weight | 1375456.1 |
